Abstract：
A novel chemoheterotrophic iron-reducing micro-organism, designated as strain LSZ-M11000T, was isolated from sediment of the Marianas Trench. Phylogenetic analysis based on the 16S rRNA gene revealed that strain LSZ-M11000T belonged to genus Tepidibacillus, with 97 % identity to that of Tepidibacillus fermentans STGHT, a mesophilic bacterium isolated from the Severo-Stavropolskoye underground gas storage facility in Russia. The polar lipid profile of strain LSZ-M11000T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, as well as other unidentified phospholipids and lipids. The major fatty acids were C16 : 0 (28.4 %), C18 : 0 (15.8 %), iso-C15 : 0 (12.9 %), and anteiso-C15 : 0 (12.0 %). Strain LSZ-M11000T had no menaquinone. Genome sequencing revealed that the genome size of strain LSZ-M11000T was 2.97 Mb and the DNA G+C content was 37.9 mol%. The average nucleotide identity values between strain LSZ-M11000T and its close phylogenetic relatives, Tepidibacillus fermentans STGHT and Tepidibacillus decaturensis Z9T, were 76.4 and 72.6 %, respectively. The corresponding DNA-DNA hybridization estimates were 20.9 and 23.4 %, respectively. Cells of strain LSZ-M11000T were rod-shaped (1.0-1.5×0.3-0.5 µm). Using pyruvate as an electron donor, it was capable of reducing KMnO4, MnO2, As(V), NaNO3, NaNO2, Na2SO4, Na2S2O3, and K2Cr2O7. Based on phenotypic, genotypic, and phylogenetic evidence, strain LSZ-M11000T is proposed to be a novel strain of the genus Tepidibacillus, for which the name Tepdibacillus marianensis is proposed. The type strain is LSZ-M11000T (=CCAM 1008T=JCM 39431T).
摘要：
一种新型的化学异养铁还原微生物，命名为菌株LSZ-M11000T，是从马里亚纳海沟的沉积物中分离出来的。基于16SrRNA基因的系统发育分析表明，菌株LSZ-M11000T属于Tepidibacillus属，与发酵体芽孢杆菌STGHT有97%的同一性，从俄罗斯的Severo-Stavropolskoye地下储气设施中分离出的一种嗜温细菌。菌株LSZ-M11000T的极性脂质谱由二磷脂酰甘油组成，磷脂酰甘油，磷脂酰乙醇胺，以及其他未知的磷脂和脂质。主要脂肪酸为C16:0(28.4%),C18：0（15.8%），iso-C15：0（12.9%），和anteiso-C15：0（12.0%）。菌株LSZ-M11000T没有甲基萘醌。基因组测序显示，菌株LSZ-M11000T的基因组大小为2.97Mb，DNAGC含量为37.9mol％。菌株LSZ-M11000T与其亲缘关系的平均核苷酸同一性值，发酵性腹泻杆菌STGHT和decaturensis性腹泻杆菌Z9T,分别为76.4%和72.6%，分别。相应的DNA-DNA杂交估计值分别为20.9%和23.4%,分别。菌株LSZ-M11000T的细胞呈杆状（1.0-1.5×0.3-0.5µm）。使用丙酮酸作为电子供体，它能够还原KMnO4，MnO2，As（V），NaNO3、NaNO2、Na2SO4、Na2S2O3和K2Cr2O7。基于表型，基因型，和系统发育证据，菌株LSZ-M11000T被认为是铁芽孢杆菌属的新菌株，为此提出了天麻杆菌的名称。菌株类型为LSZ-M11000T(=CCAM1008T=JCM39431T)。
