PDF(Pubmed)
Abstract：
UNASSIGNED: Clinical significance of coagulase-negative staphylococci (CoNS) has been gradually acknowledged in both healthcare and clinical research, but approaches for their precise discrimination at the species level remain scarce. The current study aimed to evaluate the association of CoNS with orthopedic infections, where accurate and prompt identification of etiology is crucial for appropriate diagnosis and treatment decision-making.
UNASSIGNED: A 16S rRNA-based quantitative PCR (qPCR) assay was developed for the detection of Staphylococcus genus and two panels of 3-plex qPCR assays for further differentiation of six CoNS species with remarkable clinical significance, including S. epidermidis, S. haemolyticus, S. simulans, S. hominis, S. capitis, and S. caprae. All the assays exhibited excellent analytical performance. ΔCq (quantification cycle) between 16S rRNA and CoNS species-specific targets was established to determine the primary CoNS. These methods were applied to detect CoNS in wound samples from orthopedic patients with and without infection.
UNASSIGNED: Overall, CoNS were detected in 17.8% (21/118) of patients with clinically suspected infection and in 9.8% (12/123) of patients without any infection symptom (p < 0.05). Moreover, the association with infection was found to be bacterial quantity dependent. S. epidermidis was identified as the predominant species, followed by S. simulans, S. haemolyticus, and S. hominis. Male sex, open injury, trauma, and lower extremity were determined as risk factors for CoNS infections. CoNS-positive patients had significantly longer hospitalization duration (20 days (15, 33) versus 13 days (7, 22) for Staphylococcus-negative patients, p = 0.003), which could be a considerable burden for healthcare and individual patients. Considering the complex characteristics and devastating consequences of orthopedic infections, further expanding the detection scope for CoNS may be pursued to better understand the etiology of orthopedic infections and to improve therapeutic strategies.
UNASSIGNED: A 16S rRNA-based quantitative PCR (qPCR) assay was developed for the detection of Staphylococcus genus and two panels of 3-plex qPCR assays for further differentiation of six CoNS species with remarkable clinical significance, including S. epidermidis, S. haemolyticus, S. simulans, S. hominis, S. capitis, and S. caprae. All the assays exhibited excellent analytical performance. ΔCq (quantification cycle) between 16S rRNA and CoNS species-specific targets was established to determine the primary CoNS. These methods were applied to detect CoNS in wound samples from orthopedic patients with and without infection.
UNASSIGNED: Overall, CoNS were detected in 17.8% (21/118) of patients with clinically suspected infection and in 9.8% (12/123) of patients without any infection symptom (p < 0.05). Moreover, the association with infection was found to be bacterial quantity dependent. S. epidermidis was identified as the predominant species, followed by S. simulans, S. haemolyticus, and S. hominis. Male sex, open injury, trauma, and lower extremity were determined as risk factors for CoNS infections. CoNS-positive patients had significantly longer hospitalization duration (20 days (15, 33) versus 13 days (7, 22) for Staphylococcus-negative patients, p = 0.003), which could be a considerable burden for healthcare and individual patients. Considering the complex characteristics and devastating consequences of orthopedic infections, further expanding the detection scope for CoNS may be pursued to better understand the etiology of orthopedic infections and to improve therapeutic strategies.
摘要：
■凝固酶阴性葡萄球菌（CoNS）的临床意义已在医疗保健和临床研究中逐渐得到认可，但是在物种层面上精确区分它们的方法仍然很少。目前的研究旨在评估CoNS与骨科感染的关系，准确和及时的病因识别对于适当的诊断和治疗决策至关重要。
■开发了一种基于16SrRNA的定量PCR（qPCR）测定法，用于检测葡萄球菌属，并开发了两组3-plexqPCR测定法，用于进一步区分6种具有显着临床意义的CoNS物种，包括表皮葡萄球菌,S、溶血病，S、模拟器，S、人类，S、capitis，还有S.Caprae.所有测定均表现出优异的分析性能。建立16SrRNA和CoNS物种特异性靶标之间的ΔCq(定量循环)以确定初级CoNS。这些方法用于检测来自有和没有感染的骨科患者的伤口样品中的CoNS。
■总的来说，在临床怀疑感染的患者中，有17.8％（21/118）检测到CoNS，在无任何感染症状的患者中，有9.8％（12/123）检测到CoNS（p<0.05）。此外，发现与感染的关联是细菌数量依赖性的。表皮葡萄球菌被确定为主要物种，紧随其后的是S.simulans，S、溶血病，和人类。男性,开放性损伤,创伤,和下肢被确定为CoNS感染的危险因素。CoNS阳性患者的住院时间明显更长（20天（15，33）与葡萄球菌阴性患者的13天（7，22）相比，p=0.003),这对医疗保健和个体患者来说可能是一个相当大的负担。考虑到骨科感染的复杂特征和破坏性后果，为了更好地了解骨科感染的病因并改进治疗策略,可以进一步扩大CoNS的检测范围.
■开发了一种基于16SrRNA的定量PCR（qPCR）测定法，用于检测葡萄球菌属，并开发了两组3-plexqPCR测定法，用于进一步区分6种具有显着临床意义的CoNS物种，包括表皮葡萄球菌,S、溶血病，S、模拟器，S、人类，S、capitis，还有S.Caprae.所有测定均表现出优异的分析性能。建立16SrRNA和CoNS物种特异性靶标之间的ΔCq(定量循环)以确定初级CoNS。这些方法用于检测来自有和没有感染的骨科患者的伤口样品中的CoNS。
■总的来说，在临床怀疑感染的患者中，有17.8％（21/118）检测到CoNS，在无任何感染症状的患者中，有9.8％（12/123）检测到CoNS（p<0.05）。此外，发现与感染的关联是细菌数量依赖性的。表皮葡萄球菌被确定为主要物种，紧随其后的是S.simulans，S、溶血病，和人类。男性,开放性损伤,创伤,和下肢被确定为CoNS感染的危险因素。CoNS阳性患者的住院时间明显更长（20天（15，33）与葡萄球菌阴性患者的13天（7，22）相比，p=0.003),这对医疗保健和个体患者来说可能是一个相当大的负担。考虑到骨科感染的复杂特征和破坏性后果，为了更好地了解骨科感染的病因并改进治疗策略,可以进一步扩大CoNS的检测范围.
