PDF(Pubmed)
Abstract：
UNASSIGNED: The human immunodeficiency virus (HIV) remains a critical global health issue, with a pressing need for effective diagnostic and monitoring tools.
UNASSIGNED: This study explored distinctions in salivary metabolome among healthy individuals, individuals with HIV, and those receiving highly active antiretroviral therapy (HAART). Utilizing LC-MS/MS for exhaustive metabolomics profiling, we analyzed 90 oral saliva samples from individuals with HIV, categorized by CD4 count levels in the peripheral blood.
UNASSIGNED: Orthogonal partial least squares-discriminant analysis (OPLS-DA) and other analyses underscored significant metabolic alterations in individuals with HIV, especially in energy metabolism pathways. Notably, post-HAART metabolic profiles indicated a substantial presence of exogenous metabolites and changes in amino acid pathways like arginine, proline, and lysine degradation. Key metabolites such as citric acid, L-glutamic acid, and L-histidine were identified as potential indicators of disease progression or recovery. Differential metabolite selection and functional enrichment analysis, combined with receiver operating characteristic (ROC) and random forest analyses, pinpointed potential biomarkers for different stages of HIV infection. Additionally, our research examined the interplay between oral metabolites and microorganisms such as herpes simplex virus type 1 (HSV1), bacteria, and fungi in individuals with HIV, revealing crucial interactions.
UNASSIGNED: This investigation seeks to contribute understanding into the metabolic shifts occurring in HIV infection and following the initiation of HAART, while tentatively proposing novel avenues for diagnostic and treatment monitoring through salivary metabolomics.
UNASSIGNED: This study explored distinctions in salivary metabolome among healthy individuals, individuals with HIV, and those receiving highly active antiretroviral therapy (HAART). Utilizing LC-MS/MS for exhaustive metabolomics profiling, we analyzed 90 oral saliva samples from individuals with HIV, categorized by CD4 count levels in the peripheral blood.
UNASSIGNED: Orthogonal partial least squares-discriminant analysis (OPLS-DA) and other analyses underscored significant metabolic alterations in individuals with HIV, especially in energy metabolism pathways. Notably, post-HAART metabolic profiles indicated a substantial presence of exogenous metabolites and changes in amino acid pathways like arginine, proline, and lysine degradation. Key metabolites such as citric acid, L-glutamic acid, and L-histidine were identified as potential indicators of disease progression or recovery. Differential metabolite selection and functional enrichment analysis, combined with receiver operating characteristic (ROC) and random forest analyses, pinpointed potential biomarkers for different stages of HIV infection. Additionally, our research examined the interplay between oral metabolites and microorganisms such as herpes simplex virus type 1 (HSV1), bacteria, and fungi in individuals with HIV, revealing crucial interactions.
UNASSIGNED: This investigation seeks to contribute understanding into the metabolic shifts occurring in HIV infection and following the initiation of HAART, while tentatively proposing novel avenues for diagnostic and treatment monitoring through salivary metabolomics.
摘要：
■人类免疫缺陷病毒（HIV）仍然是一个至关重要的全球健康问题，迫切需要有效的诊断和监测工具。
■这项研究探索了健康个体唾液代谢组的区别，艾滋病毒感染者，和那些接受高活性抗逆转录病毒治疗（HAART）。利用LC-MS/MS进行详尽的代谢组学分析，我们分析了90例HIV感染者的口腔唾液样本,根据外周血中的CD4计数水平进行分类。
■正交偏最小二乘判别分析（OPLS-DA）和其他分析强调了HIV感染者的显着代谢改变，特别是在能量代谢途径中。值得注意的是,后HAART代谢谱表明大量存在外源代谢物和氨基酸途径的变化，如精氨酸，脯氨酸,和赖氨酸降解。关键代谢产物如柠檬酸,L-谷氨酸,和L-组氨酸被鉴定为疾病进展或恢复的潜在指标。差异代谢物选择和功能富集分析，结合接收机工作特性(ROC)和随机森林分析，确定HIV感染不同阶段的潜在生物标志物。此外,我们的研究检查了口腔代谢物和微生物之间的相互作用，如单纯疱疹病毒1型（HSV1），细菌,和感染艾滋病毒的人身上的真菌，揭示关键的互动。
■本调查旨在帮助了解HIV感染和HAART开始后发生的代谢变化，同时初步提出了通过唾液代谢组学进行诊断和治疗监测的新途径。
■这项研究探索了健康个体唾液代谢组的区别，艾滋病毒感染者，和那些接受高活性抗逆转录病毒治疗（HAART）。利用LC-MS/MS进行详尽的代谢组学分析，我们分析了90例HIV感染者的口腔唾液样本,根据外周血中的CD4计数水平进行分类。
■正交偏最小二乘判别分析（OPLS-DA）和其他分析强调了HIV感染者的显着代谢改变，特别是在能量代谢途径中。值得注意的是,后HAART代谢谱表明大量存在外源代谢物和氨基酸途径的变化，如精氨酸，脯氨酸,和赖氨酸降解。关键代谢产物如柠檬酸,L-谷氨酸,和L-组氨酸被鉴定为疾病进展或恢复的潜在指标。差异代谢物选择和功能富集分析，结合接收机工作特性(ROC)和随机森林分析，确定HIV感染不同阶段的潜在生物标志物。此外,我们的研究检查了口腔代谢物和微生物之间的相互作用，如单纯疱疹病毒1型（HSV1），细菌,和感染艾滋病毒的人身上的真菌，揭示关键的互动。
■本调查旨在帮助了解HIV感染和HAART开始后发生的代谢变化，同时初步提出了通过唾液代谢组学进行诊断和治疗监测的新途径。
