PDF(Pubmed)
Abstract：
UNASSIGNED: We assessed the in vitro anti-chlamydial activity of fresh vaginal secretions, deciphering the microbial and metabolic components able to counteract Chlamydia trachomatis viability.
UNASSIGNED: Forty vaginal samples were collected from a group of reproductive-aged women and their anti-chlamydial activity was evaluated by inhibition experiments. Each sample underwent 16S rRNA metabarcoding sequencing to determine the bacterial composition, as well as 1H-NMR spectroscopy to detect and quantify the presence of vaginal metabolites.
UNASSIGNED: Samples characterized by a high anti-chlamydial activity were enriched in Lactobacillus, especially Lactobacillus crispatus and Lactobacillus iners, while not-active samples exhibited a significant reduction of lactobacilli, along with higher relative abundances of Streptococcus and Olegusella. Lactobacillus gasseri showed an opposite behavior compared to L. crispatus, being more prevalent in not-active vaginal samples. Higher concentrations of several amino acids (i.e., isoleucine, leucine, and aspartate; positively correlated to the abundance of L. crispatus and L. jensenii) lactate, and 4-aminobutyrate were the most significant metabolic fingerprints of highly active samples. Acetate and formate concentrations, on the other hand, were related to the abundances of a group of anaerobic opportunistic bacteria (including Prevotella, Dialister, Olegusella, Peptostreptococcus, Peptoniphilus, Finegoldia and Anaerococcus). Finally, glucose, correlated to Streptococcus, Lachnospira and Alloscardovia genera, emerged as a key molecule of the vaginal environment: indeed, the anti-chlamydial effect of vaginal fluids decreased as glucose concentrations increased.
UNASSIGNED: These findings could pave the way for novel strategies in the prevention and treatment of chlamydial urogenital infections, such as lactobacilli probiotic formulations or lactobacilli-derived postbiotics.
UNASSIGNED: Forty vaginal samples were collected from a group of reproductive-aged women and their anti-chlamydial activity was evaluated by inhibition experiments. Each sample underwent 16S rRNA metabarcoding sequencing to determine the bacterial composition, as well as 1H-NMR spectroscopy to detect and quantify the presence of vaginal metabolites.
UNASSIGNED: Samples characterized by a high anti-chlamydial activity were enriched in Lactobacillus, especially Lactobacillus crispatus and Lactobacillus iners, while not-active samples exhibited a significant reduction of lactobacilli, along with higher relative abundances of Streptococcus and Olegusella. Lactobacillus gasseri showed an opposite behavior compared to L. crispatus, being more prevalent in not-active vaginal samples. Higher concentrations of several amino acids (i.e., isoleucine, leucine, and aspartate; positively correlated to the abundance of L. crispatus and L. jensenii) lactate, and 4-aminobutyrate were the most significant metabolic fingerprints of highly active samples. Acetate and formate concentrations, on the other hand, were related to the abundances of a group of anaerobic opportunistic bacteria (including Prevotella, Dialister, Olegusella, Peptostreptococcus, Peptoniphilus, Finegoldia and Anaerococcus). Finally, glucose, correlated to Streptococcus, Lachnospira and Alloscardovia genera, emerged as a key molecule of the vaginal environment: indeed, the anti-chlamydial effect of vaginal fluids decreased as glucose concentrations increased.
UNASSIGNED: These findings could pave the way for novel strategies in the prevention and treatment of chlamydial urogenital infections, such as lactobacilli probiotic formulations or lactobacilli-derived postbiotics.
摘要：
■我们评估了新鲜阴道分泌物的体外抗衣原体活性，破译能够抵消沙眼衣原体活力的微生物和代谢成分。
■从一组育龄妇女中收集了40个阴道样品，并通过抑制实验评估了它们的抗衣原体活性。每个样品进行16SrRNA元编码测序，以确定细菌组成，以及1H-NMR光谱来检测和定量阴道代谢物的存在。
■具有高抗衣原体活性的样品富含乳杆菌，尤其是卷曲乳杆菌和乳杆菌,虽然非活性样品表现出乳杆菌的显着减少，以及较高的相对丰度的链球菌和Olegusella。与卷曲乳杆菌相比，gasseri乳杆菌表现出相反的行为，在不活跃的阴道样本中更为普遍。较高浓度的几种氨基酸(即，异亮氨酸,亮氨酸,和天冬氨酸；与crispatus和L.jensenii）乳酸的丰度呈正相关，4-氨基丁酸酯是高活性样品中最重要的代谢指纹图谱。乙酸盐和甲酸盐浓度，另一方面,与一组厌氧机会细菌(包括普雷沃氏菌，Dialister,Olegusella,肽链球菌,Peptoniphilus,Finegoldia和厌氧球菌）。最后,葡萄糖，与链球菌相关，落叶螺旋体和异型卡多维亚属，作为阴道环境的关键分子出现:事实上，阴道液的抗衣原体作用随着葡萄糖浓度的增加而降低.
■这些发现可能为预防和治疗衣原体泌尿生殖道感染的新策略铺平道路。例如乳杆菌益生菌制剂或乳杆菌衍生的益生菌。
■从一组育龄妇女中收集了40个阴道样品，并通过抑制实验评估了它们的抗衣原体活性。每个样品进行16SrRNA元编码测序，以确定细菌组成，以及1H-NMR光谱来检测和定量阴道代谢物的存在。
■具有高抗衣原体活性的样品富含乳杆菌，尤其是卷曲乳杆菌和乳杆菌,虽然非活性样品表现出乳杆菌的显着减少，以及较高的相对丰度的链球菌和Olegusella。与卷曲乳杆菌相比，gasseri乳杆菌表现出相反的行为，在不活跃的阴道样本中更为普遍。较高浓度的几种氨基酸(即，异亮氨酸,亮氨酸,和天冬氨酸；与crispatus和L.jensenii）乳酸的丰度呈正相关，4-氨基丁酸酯是高活性样品中最重要的代谢指纹图谱。乙酸盐和甲酸盐浓度，另一方面,与一组厌氧机会细菌(包括普雷沃氏菌，Dialister,Olegusella,肽链球菌,Peptoniphilus,Finegoldia和厌氧球菌）。最后,葡萄糖，与链球菌相关，落叶螺旋体和异型卡多维亚属，作为阴道环境的关键分子出现:事实上，阴道液的抗衣原体作用随着葡萄糖浓度的增加而降低.
■这些发现可能为预防和治疗衣原体泌尿生殖道感染的新策略铺平道路。例如乳杆菌益生菌制剂或乳杆菌衍生的益生菌。
