PDF(Pubmed)
Abstract：
Due to their ability to replicate the in vivo microenvironment through cell interaction and induce cells to stimulate cell function, three-dimensional cell culture models can overcome the limitations of two-dimensional models. Organoids are 3D models that demonstrate the ability to replicate the natural structure of an organ. In most organoid tissue cultures, matrigel made of a mouse tumor extracellular matrix protein mixture is an essential ingredient. However, its tumor-derived origin, batch-to-batch variation, high cost, and safety concerns have limited the usefulness of organoid drug development and regenerative medicine. Its clinical application has also been hindered by the fact that organoid generation is dependent on the use of poorly defined matrices. Therefore, matrix optimization is a crucial step in developing organoid culture that introduces alternatives as different materials. Recently, a variety of substitute materials has reportedly replaced matrigel. The purpose of this study is to review the significance of the latest advances in materials for cell culture applications and how they enhance build network systems by generating proper cell behavior. Excellence in cell behavior is evaluated from their cell characteristics, cell proliferation, cell differentiation, and even gene expression. As a result, graphene oxide as a matrix optimization demonstrated high potency in developing organoid models. Graphene oxide can promote good cell behavior and is well known for having good biocompatibility. Hence, advances in matrix optimization of graphene oxide provide opportunities for the future development of advanced organoid models.
摘要：
由于它们能够通过细胞相互作用复制体内微环境并诱导细胞刺激细胞功能，三维细胞培养模型可以克服二维模型的局限性。类器官是3D模型，展示了复制器官自然结构的能力。在大多数类器官组织培养中，由小鼠肿瘤细胞外基质蛋白混合物制成的基质胶是必需成分。然而,它的肿瘤来源，批次到批次的变化，高成本,和安全性问题限制了类器官药物开发和再生医学的有用性。它的临床应用也受到以下事实的阻碍:类器官的产生依赖于使用不清楚定义的基质。因此,基质优化是开发类器官培养的关键步骤，它将替代品引入不同的材料。最近,据报道，各种替代材料已经取代了基质胶。这项研究的目的是回顾细胞培养应用材料的最新进展的意义，以及它们如何通过产生适当的细胞行为来增强网络系统的构建。从细胞特性评估细胞行为的卓越，细胞增殖，细胞分化，甚至基因表达。因此,氧化石墨烯作为基质优化在开发类器官模型中表现出很高的效力。氧化石墨烯可以促进良好的细胞行为，并且众所周知具有良好的生物相容性。因此，氧化石墨烯矩阵优化的进展为未来开发先进的类器官模型提供了机会。
