UNASSIGNED: In this presented study, DPSCs were cultured and cell characterizations were performed by using flow cytometry and immunostainings. Bilateral mental nerve injury models of rabbits were created. In the control group (n = 10), saline was applied, and in the study group (n = 10), 2 × 106 DPSCs were applied to the repaired nerve areas. After 3 weeks, animals were killed and histological examination was obtained by using Masson\'s trichrome staining. An unpaired Student\'s t test was used when comparing the groups. Differences were considered to be statistically significant at P values of less than 0.05.
UNASSIGNED: The DPSCs demonstrated a homogeneous population of mesenchymal stromal cells which expressed cluster of differentiation CD44, CD73, CD90, and CD105 and lack of CD34, CD45, and HLA-DR. Our finding clearly demonstrated that a lower number of cross-sectioned axons were founded in the control group (60.18 ± 2.52) compared to the study group (72.96 ± 2.43) (p = 0.00).
UNASSIGNED: DPSCs promote mental nerve axonal regeneration. These results suggest that DPSCs provide an important accessible source of adult stem cells for mental nerve regeneration.
■在这项研究中,培养DPSC并通过使用流式细胞术和免疫染色进行细胞表征。建立兔双侧精神神经损伤模型。在对照组(n=10)中,使用生理盐水,在研究组(n=10)中,将2×106个DPSC应用于修复的神经区域。3周后,处死动物,并使用Masson三色染色进行组织学检查。当比较各组时,使用非配对学生t检验。在P值小于0.05时,认为差异具有统计学意义。
■DPSC表现出间充质基质细胞的同质群体,其表达分化CD44,CD73,CD90和CD105的簇,并且缺乏CD34,CD45和HLA-DR。我们的发现清楚地表明,与研究组(72.96±2.43)相比,对照组(60.18±2.52)的横截面轴突数量较少(p=0.00)。
■DPSC促进精神神经轴突再生。这些结果表明,DPSC为精神神经再生提供了重要的成人干细胞来源。