PDF(Pubmed)
Abstract：
UNASSIGNED: The mental nerve, the extended part of the inferior alveolar nerve, is often injured during dentoalveolar, orthognathic, or tumor surgery. Numerous therapeutic interventions, including surgery and pharmacotherapy, have been used to enhance the recovery of nerve injuries. Dental pulp stem cells (DPSCs) represent an easily accessible source of adult stem cells that can be isolated from the pulp of extracted teeth. This study evaluated the effect of DPSCs on the regeneration of the mental nerve injury model of rabbits.
UNASSIGNED: In this presented study, DPSCs were cultured and cell characterizations were performed by using flow cytometry and immunostainings. Bilateral mental nerve injury models of rabbits were created. In the control group (n = 10), saline was applied, and in the study group (n = 10), 2 × 106 DPSCs were applied to the repaired nerve areas. After 3 weeks, animals were killed and histological examination was obtained by using Masson\'s trichrome staining. An unpaired Student\'s t test was used when comparing the groups. Differences were considered to be statistically significant at P values of less than 0.05.
UNASSIGNED: The DPSCs demonstrated a homogeneous population of mesenchymal stromal cells which expressed cluster of differentiation CD44, CD73, CD90, and CD105 and lack of CD34, CD45, and HLA-DR. Our finding clearly demonstrated that a lower number of cross-sectioned axons were founded in the control group (60.18 ± 2.52) compared to the study group (72.96 ± 2.43) (p = 0.00).
UNASSIGNED: DPSCs promote mental nerve axonal regeneration. These results suggest that DPSCs provide an important accessible source of adult stem cells for mental nerve regeneration.
UNASSIGNED: In this presented study, DPSCs were cultured and cell characterizations were performed by using flow cytometry and immunostainings. Bilateral mental nerve injury models of rabbits were created. In the control group (n = 10), saline was applied, and in the study group (n = 10), 2 × 106 DPSCs were applied to the repaired nerve areas. After 3 weeks, animals were killed and histological examination was obtained by using Masson\'s trichrome staining. An unpaired Student\'s t test was used when comparing the groups. Differences were considered to be statistically significant at P values of less than 0.05.
UNASSIGNED: The DPSCs demonstrated a homogeneous population of mesenchymal stromal cells which expressed cluster of differentiation CD44, CD73, CD90, and CD105 and lack of CD34, CD45, and HLA-DR. Our finding clearly demonstrated that a lower number of cross-sectioned axons were founded in the control group (60.18 ± 2.52) compared to the study group (72.96 ± 2.43) (p = 0.00).
UNASSIGNED: DPSCs promote mental nerve axonal regeneration. These results suggest that DPSCs provide an important accessible source of adult stem cells for mental nerve regeneration.
摘要：
■精神神经,下牙槽神经的延伸部分，经常在牙槽骨时受伤，正颌,或者肿瘤手术.许多治疗干预措施，包括手术和药物治疗,已被用于增强神经损伤的恢复。牙髓干细胞(DPSC)代表可从拔牙牙髓分离的成体干细胞的容易获得的来源。本研究评估了DPSCs对兔精神神经损伤模型再生的影响。
■在这项研究中，培养DPSC并通过使用流式细胞术和免疫染色进行细胞表征。建立兔双侧精神神经损伤模型。在对照组（n=10）中，使用生理盐水，在研究组(n=10)中，将2×106个DPSC应用于修复的神经区域。3周后,处死动物，并使用Masson三色染色进行组织学检查。当比较各组时,使用非配对学生t检验。在P值小于0.05时,认为差异具有统计学意义。
■DPSC表现出间充质基质细胞的同质群体，其表达分化CD44，CD73，CD90和CD105的簇，并且缺乏CD34，CD45和HLA-DR。我们的发现清楚地表明，与研究组（72.96±2.43）相比，对照组（60.18±2.52）的横截面轴突数量较少（p=0.00）。
■DPSC促进精神神经轴突再生。这些结果表明，DPSC为精神神经再生提供了重要的成人干细胞来源。
■在这项研究中，培养DPSC并通过使用流式细胞术和免疫染色进行细胞表征。建立兔双侧精神神经损伤模型。在对照组（n=10）中，使用生理盐水，在研究组(n=10)中，将2×106个DPSC应用于修复的神经区域。3周后,处死动物，并使用Masson三色染色进行组织学检查。当比较各组时,使用非配对学生t检验。在P值小于0.05时,认为差异具有统计学意义。
■DPSC表现出间充质基质细胞的同质群体，其表达分化CD44，CD73，CD90和CD105的簇，并且缺乏CD34，CD45和HLA-DR。我们的发现清楚地表明，与研究组（72.96±2.43）相比，对照组（60.18±2.52）的横截面轴突数量较少（p=0.00）。
■DPSC促进精神神经轴突再生。这些结果表明，DPSC为精神神经再生提供了重要的成人干细胞来源。
