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Abstract：
First-void urine (FVU) samples, containing human papillomavirus (HPV)-specific IgG from female genital tract secretions, provide a non-invasive option for disease monitoring and vaccine impact assessment. This study explores the utility of FVU for IgG quantification, exploring stability and compatibility with DNA preservation methods, alongside various IgG enrichment methods. Healthy female volunteers provided FVU and serum samples. FVU was collected with or without urine conservation medium (UCM) and stored under different conditions before freezing at -80 °C. Four IgG enrichment methods were tested on FVU samples. All samples were analyzed using three total human IgG quantification assays and an in-house HPV16-specific IgG assay. Samples stored with UCM buffer had higher total and HPV16-specific IgG concentrations (p ≤ 0.01) and IgG remained stable for at least 14 days at room temperature. Among IgG enrichment methods, Amicon filtration (AM) and AM combined with Melon Gel purification (AM-MG) provided similar HPV16-IgG concentrations, correlating strongly with serum levels. Protein G magnetic beads methods were incompatible with time-resolved fluorescence-based assays. This study highlights FVU as a reliable and convenient sample for IgG quantification, demonstrating stability for at least 14 days at room temperature and compatibility with UCM DNA preservation. It emphasizes the need to select appropriate IgG enrichment methods and confirms the suitability of both AM and AM-MG methods, with a slightly better performance for AM-MG.
摘要：
首次尿液（FVU）样本，含有来自女性生殖道分泌物的人乳头瘤病毒（HPV）特异性IgG，为疾病监测和疫苗影响评估提供非侵入性选择。本研究探讨了FVU用于IgG定量的实用性，探索DNA保存方法的稳定性和兼容性，以及各种IgG富集方法。健康女性志愿者提供FVU和血清样品。在有或没有尿液保存培养基（UCM）的情况下收集FVU，并在-80°C冷冻之前在不同条件下储存。在FVU样品上测试了四种IgG富集方法。使用三个总人IgG定量测定和内部HPV16特异性IgG测定分析所有样品。用UCM缓冲液储存的样品具有较高的总和HPV16特异性IgG浓度(p≤0.01),并且IgG在室温下保持稳定至少14天。在IgG富集方法中，Amicon过滤（AM）和AM结合甜瓜凝胶纯化（AM-MG）提供了相似的HPV16-IgG浓度，与血清水平密切相关。蛋白G磁珠方法与基于时间分辨荧光的测定不相容。这项研究强调FVU作为IgG定量的可靠和方便的样品，证明在室温下至少14天的稳定性以及与UCMDNA保存的兼容性。它强调需要选择合适的IgG富集方法，并确认AM和AM-MG方法的适用性，与一个稍微更好的AM-MG性能。
