Abstract：
This research performed cellulase production by Aspergillus fumigatus A4112 and evaluated its potential use in palm oil mill effluent (POME) hydrolysis to recover oil simultaneously with the generation of fermentable sugar useful for biofuel production under non-sterilized conditions. Empty fruit bunch (EFB) without pretreatment was used as carbon source. The combination of nitrogen sources facilitated CMCase production. The maximum activity (3.27 U/mL) was obtained by 1.0 g/L peptone and 1.5 g/L (NH4)2SO4 and 20 g/L EFB at 40 °C for 7 days. High level of FPase activity (39.51 U/mL) was also obtained. Interestingly, the enzyme retained its cellulase activities more than 60% at ambient temperature over 15 days. In enzymatic hydrolysis, Triton X-100 was an effective surfactant to increase total oil recovery in the floating form. High yield of reducing sugar (50.13 g/L) and 21% (v/v) of floating oil was recoverable at 65 °C for 48 h. Methane content of the raw POME increased from 41.49 to 64.94% by using de-oiled POME hydrolysate which was higher than using the POME hydrolysate (59.82%). The results demonstrate the feasibility of the constructed process for oil recovery coupled with a subsequent step for methane yield enhancement in biogas production process that benefits the palm oil industry.
摘要：
这项研究通过烟曲霉A4112进行了纤维素酶的生产，并评估了其在棕榈油厂废水（POME）水解中的潜在用途，以在非灭菌条件下同时产生可用于生物燃料生产的可发酵糖。未经预处理的空果束（EFB）用作碳源。氮源的组合促进了CMCase的生产。通过1.0g/L蛋白ept和1.5g/L（NH4）2SO4和20g/LEFB在40°C下持续7天获得最大活性（3.27U/mL）。还获得高水平的FPase活性(39.51U/mL)。有趣的是,该酶在环境温度下保持其纤维素酶活性超过60％超过15天。在酶水解中，TritonX-100是一种有效的表面活性剂，可以提高漂浮形式的总油采收率。在65°C下48小时可回收高产率的还原糖（50.13g/L）和21％（v/v）的浮油。通过使用脱油POME水解产物，原始POME的甲烷含量从41.49增加到64.94％，高于使用POME水解产物（59.82％）。结果表明，所构建的采油工艺与沼气生产过程中甲烷产量提高的后续步骤相结合是可行的，这有利于棕榈油行业。
