PDF(Pubmed)
Abstract：
Molecular identification of pollen carried by insects informs about their history of visited plants. For migratory butterflies, it can be used to trace long-range movements enduring days of flight over thousands of kilometers. Here, we present a protocol to (1) isolate pollen grains from butterfly bodies and (2) prepare metabarcoding libraries for their identification using the internal transcribed spacer 2 fragment, a common barcode used to identify plants. This protocol would be applicable to other insect groups and metabarcoding markers. For complete details on the use and execution of this protocol, please refer to Suchan et al.1 and Gorki et al.2.
摘要：
昆虫携带花粉的分子鉴定可告知其参观植物的历史。对于迁徙的蝴蝶，它可以用来追踪数千公里飞行中的远距离运动。这里,我们提出了一个协议（1）从蝴蝶体中分离花粉粒和（2）准备他们的鉴定使用内部转录的间隔区2片段的metabarcoding文库，用于识别植物的普通条形码。该协议将适用于其他昆虫群体和元编码标记。有关此协议的使用和执行的完整详细信息，请参考Suchanetal.1和Gorkietal.2。
