Abstract：
In vitro selection of allosteric ribozymes has many challenges, such as complex and time-consuming experimental procedures, uncertain results, and the unwanted functionality of the enriched sequences. The precise computational design of allosteric ribozymes is achievable using RNA secondary structure folding principles. The computational design of allosteric ribozymes is based on experimentally validated EAs, random search algorithms, and a partition function for RNA folding. The in silico design achieves an accuracy exceeding 90%. Various algorithms with different logic gates have been automated via computer programs that can quickly create many allosteric sequences. This can eliminate the need for in vitro selection of allosteric ribozymes, thus vastly reducing the time and cost required.
摘要：
体外选择变构核酶有许多挑战，例如复杂而耗时的实验程序，不确定的结果，和富集序列的不想要的功能。使用RNA二级结构折叠原理可以实现变构核酶的精确计算设计。变构核酶的计算设计基于实验验证的EA，随机搜索算法，和RNA折叠的分配函数。在硅设计达到了超过90%的精度。具有不同逻辑门的各种算法已经通过能够快速创建许多变构序列的计算机程序自动化。这可以消除体外选择变构核酶的需要，从而大大减少了所需的时间和成本。
