Abstract：
RT-LAMP is an effective alternative to RT-PCR-based diagnostics, offering high specificity, sensitivity, and rapid results. One notable advantage is the robustness of its enzymes, allowing for direct amplification from crude samples without the need for prior isolation of RNA. Colorimetric LAMP is particularly attractive as it eliminates the need for complex instrumentation, making it suitable for point-of-care applications. Here, we present a comprehensive step-by-step protocol for establishing an RT-LAMP-based test for direct detection of SARS-CoV-2 genomic RNA in saliva samples using different colorimetric detection methods. Importantly, this versatile test can be easily adapted to detect emerging pathogens.
摘要：
RT-LAMP是基于RT-PCR的诊断的有效替代方案，提供高特异性，灵敏度,和快速的结果。一个显著的优点是它的酶的鲁棒性，允许从粗样品中直接扩增，而不需要事先分离RNA。比色LAMP特别有吸引力，因为它消除了对复杂仪器的需求，使其适合点的护理应用。这里,我们提出了一个全面的分步方案,用于建立基于RT-LAMP的测试,使用不同的比色检测方法直接检测唾液样品中的SARS-CoV-2基因组RNA.重要的是,这种多功能测试可以很容易地适应检测新出现的病原体。
