Abstract：
Burns are a global health problem and can be caused by several factors, including ultraviolet (UV) radiation. Exposure to UVB radiation can cause sunburn and a consequent inflammatory response characterised by pain, oedema, inflammatory cell infiltration, and erythema. Pharmacological treatments available to treat burns and the pain caused by them include nonsteroidal anti-inflammatory drugs (NSAIDs), opioids, antimicrobials and glucocorticoids, which are associated with adverse effects. Therefore, the search for new therapeutic alternatives is needed. Diosmetin, an aglycone of the flavonoid diosmin, has antinociceptive, antioxidant and anti-inflammatory properties. Thus, we evaluated the antinociceptive and anti-inflammatory effects of topical diosmetin (0.01, 0.1 and 1%) in a UVB radiation-induced sunburn model in mice. The right hind paw of the anaesthetised mice was exposed only once to UVB radiation (0.75 J/cm2) and immediately treated with diosmetin once a day for 5 days. The diosmetin antinociceptive effect was evaluated by mechanical allodynia and pain affective-motivational behaviour, while its anti-inflammatory activity was assessed by measuring paw oedema and polymorphonuclear cell infiltration. Mice exposed to UVB radiation presented mechanical allodynia, increased pain affective-motivational behaviour, paw oedema and polymorphonuclear cell infiltration into the paw tissue. Topical Pemulen® TR2 1% diosmetin reduced the mechanical allodynia, the pain affective-motivational behaviour, the paw oedema and the number of polymorphonuclear cells in the mice\'s paw tissue similar to that presented by Pemulen® TR2 0.1% dexamethasone. These findings indicate that diosmetin has therapeutic potential and may be a promising strategy for treating patients experiencing inflammatory pain, especially those associated with sunburn.
摘要：
烧伤是一个全球性的健康问题，可能由几个因素引起，包括紫外线（UV）辐射。暴露于UVB辐射可导致晒伤和随之而来的炎症反应，以疼痛为特征，水肿,炎性细胞浸润，和红斑.可用于治疗烧伤及其引起的疼痛的药物治疗包括非甾体抗炎药（NSAIDs），阿片类药物,抗生素和糖皮质激素，这与副作用有关。因此,需要寻找新的治疗替代方案。Diosmetin,黄酮类地奥司明的糖苷配基，具有抗伤害性，抗氧化和抗炎特性。因此，在UVB辐射诱导的小鼠晒伤模型中,我们评估了局部使用地美汀(0.01,0.1和1%)的镇痛和抗炎作用.将麻醉的小鼠的右后爪仅暴露于UVB辐射(0.75J/cm2)一次,并立即用薯片美素每天处理一次,持续5天。通过机械异常性疼痛和疼痛情感动机行为评估了薯片的抗伤害作用。通过测量爪水肿和多形核细胞浸润来评估其抗炎活性。暴露于UVB辐射的小鼠表现出机械性异常疼痛，增加疼痛情感激励行为，爪水肿和多形核细胞浸润到爪组织中。外用Pemuleen®TR21%的Diosmetin降低了机械性异常性疼痛，痛苦的情感激励行为，小鼠爪组织中的爪水肿和多形核细胞数量与0.1%地塞米松Pemulen®TR2的相似。这些研究结果表明，薯片具有治疗潜力，可能是治疗炎性疼痛患者的一种有希望的策略。尤其是那些与晒伤有关的.
