PDF(Pubmed)
Abstract：
BACKGROUND: Dietary fiber (DF) consumption was reported to improve insulin sensitivity, change the tryptophan metabolism, and alter the gut microbiota. Herein, this study aimed to investigate the effects of DF consumption on insulin sensitivity, tryptophan metabolism, and gut microbiota composition in sows during late pregnancy, and explore the relationship between tryptophan metabolites and insulin sensitivity regulated by DF supplementation.
RESULTS: Twelve sows were randomly assigned to two dietary treatment groups (six/group): the low-fiber (LF) group, which was fed a basal diet, and the high-fiber (HF) group, which was fed the basal diet supplemented with 22.60 g/kg inulin and 181.60 g/kg cellulose. During late pregnancy, meal test, glucose tolerance test, and insulin challenge test were used to investigate the insulin sensitivity of sows, using the percutaneous brachiocephalic vein catheterization technique. High DF consumption resulted in improved insulin sensitivity, especially during the second and third trimesters, and promoted serotonin production from tryptophan. Additionally, plasma serotonin concentration was positively correlated with the insulin sensitivity index during late pregnancy. Moreover, DF consumption elevated fecal short-chain fatty acid (SCFA) concentrations, altered fecal microbial diversity, and increased the abundances of Rikenellaceae_RC9_gut_group, Alloprevotella, Parabacteroides, Roseburia, and Sphaerochaeta, which were positively correlated to plasma serotonin concentration.
CONCLUSIONS: DF consumption improved insulin sensitivity during late pregnancy in sows, which improved microbial diversity in fecal samples and increased fecal SCFA concentrations, resulting in a positive correlation with plasma serotonin level.
RESULTS: Twelve sows were randomly assigned to two dietary treatment groups (six/group): the low-fiber (LF) group, which was fed a basal diet, and the high-fiber (HF) group, which was fed the basal diet supplemented with 22.60 g/kg inulin and 181.60 g/kg cellulose. During late pregnancy, meal test, glucose tolerance test, and insulin challenge test were used to investigate the insulin sensitivity of sows, using the percutaneous brachiocephalic vein catheterization technique. High DF consumption resulted in improved insulin sensitivity, especially during the second and third trimesters, and promoted serotonin production from tryptophan. Additionally, plasma serotonin concentration was positively correlated with the insulin sensitivity index during late pregnancy. Moreover, DF consumption elevated fecal short-chain fatty acid (SCFA) concentrations, altered fecal microbial diversity, and increased the abundances of Rikenellaceae_RC9_gut_group, Alloprevotella, Parabacteroides, Roseburia, and Sphaerochaeta, which were positively correlated to plasma serotonin concentration.
CONCLUSIONS: DF consumption improved insulin sensitivity during late pregnancy in sows, which improved microbial diversity in fecal samples and increased fecal SCFA concentrations, resulting in a positive correlation with plasma serotonin level.
摘要：
背景：据报道，食用膳食纤维（DF）可以改善胰岛素敏感性，改变色氨酸代谢，并改变肠道微生物群。在这里，本研究旨在探讨DF消耗对胰岛素敏感性的影响，色氨酸代谢,和妊娠晚期母猪的肠道菌群组成，并探讨补充DF调节的色氨酸代谢产物与胰岛素敏感性的关系。
结果：12头母猪被随机分配到两个饮食处理组（六个/组）：低纤维（LF）组，以基础饮食喂养，和高纤维(HF)组，饲喂基础日粮中添加22.60g/kg菊粉和181.60g/kg纤维素。在怀孕后期,膳食测试，葡萄糖耐量试验,和胰岛素激发试验用于调查母猪的胰岛素敏感性，采用经皮头臂静脉置管技术。高DF消耗导致改善胰岛素敏感性，尤其是在第二和第三个三个月，并促进色氨酸产生5-羟色胺。此外,妊娠晚期血浆5-羟色胺浓度与胰岛素敏感性指数呈正相关。此外，DF消耗量升高粪便短链脂肪酸（SCFA）浓度，改变粪便微生物多样性，并增加了Rikenellaceae_RC9_gut_组的丰度，Alloprevotella,副杆菌属,罗斯布里亚,和Sphaerochaeta,与血浆5-羟色胺浓度呈正相关。
结论：食用DF可改善母猪妊娠后期的胰岛素敏感性，这改善了粪便样品中的微生物多样性并增加了粪便SCFA浓度，与血浆5-羟色胺水平呈正相关。
结果：12头母猪被随机分配到两个饮食处理组（六个/组）：低纤维（LF）组，以基础饮食喂养，和高纤维(HF)组，饲喂基础日粮中添加22.60g/kg菊粉和181.60g/kg纤维素。在怀孕后期,膳食测试，葡萄糖耐量试验,和胰岛素激发试验用于调查母猪的胰岛素敏感性，采用经皮头臂静脉置管技术。高DF消耗导致改善胰岛素敏感性，尤其是在第二和第三个三个月，并促进色氨酸产生5-羟色胺。此外,妊娠晚期血浆5-羟色胺浓度与胰岛素敏感性指数呈正相关。此外，DF消耗量升高粪便短链脂肪酸（SCFA）浓度，改变粪便微生物多样性，并增加了Rikenellaceae_RC9_gut_组的丰度，Alloprevotella,副杆菌属,罗斯布里亚,和Sphaerochaeta,与血浆5-羟色胺浓度呈正相关。
结论：食用DF可改善母猪妊娠后期的胰岛素敏感性，这改善了粪便样品中的微生物多样性并增加了粪便SCFA浓度，与血浆5-羟色胺水平呈正相关。
