METHODS: We used RT‒PCR to assess the expression levels of lncRNA ATP2B2-IT2 and vascular endothelial growth factor (VEGF) in HRMECs under normal glucose (5.5 mmol/L) and high glucose (30 mmol/L) conditions. HRMECs were subsequently divided into four groups: the normal glucose (NG), high glucose (HG), high glucose with lncRNA ATP2B2-IT2 silencing (HG + si-lncRNA ATP2B2-IT2), and high glucose with silencing control (HG + si-NC) groups. The expression levels of the lncRNA ATP2B2-IT2 and VEGF in each group were determined using RT‒PCR. Thereafter, cell proliferation, migration, and neovascularization were assessed using CCK-8, Transwell, and tube formation assays, respectively.
RESULTS: RT‒PCR revealed that the expression levels of the lncRNA ATP2B2-IT2 and VEGF were greater in the HG group than in the NG group (P < 0.05). After silencing of the lncRNA ATP2B2-IT2, the expression of VEGF decreased significantly (P < 0.05). Subsequent CCK-8, Transwell, and tube formation assays demonstrated that compared to those in the NG group, the HRMECs in the HG group exhibited significantly increased proliferation, migration, and neovascularization (P < 0.05). However, after silencing of the lncRNA ATP2B2-IT2, the proliferation, migration, and neovascularization of HRMECs were significantly decreased in the HG + si-lncRNA ATP2B2-IT2 group compared to those in the HG group (P < 0.05).
CONCLUSIONS: LncRNA ATP2B2-IT2 may promote the proliferation, migration and neovascularization of HRMECs under high-glucose conditions.
方法:我们使用RT-PCR评估了正常葡萄糖(5.5mmol/L)和高糖(30mmol/L)条件下HRMEC中lncRNAATP2B2-IT2和血管内皮生长因子(VEGF)的表达水平。随后将HRMEC分为四组:正常葡萄糖(NG),高葡萄糖(HG),高葡萄糖与lncRNAATP2B2-IT2沉默(HGsi-lncRNAATP2B2-IT2),和高糖沉默对照组(HG+si-NC)。使用RT-PCR测定各组中lncRNAATP2B2-IT2和VEGF的表达水平。此后,细胞增殖,迁移,使用CCK-8,Transwell,和试管形成测定,分别。
结果:RT-PCR显示,HG组lncRNAATP2B2-IT2和VEGF的表达水平高于NG组(P<0.05)。lncRNAATP2B2-IT2沉默后,VEGF的表达显著降低(P<0.05)。随后的CCK-8,Transwell,和试管形成试验表明,与NG组相比,HG组的HRMEC表现出显著增加的增殖,迁移,新生血管形成(P<0.05)。然而,lncRNAATP2B2-IT2沉默后,增殖,迁移,与HG组相比,HGsi-lncRNAATP2B2-IT2组的HRMECs新生血管明显减少(P<0.05)。
结论:LncRNAATP2B2-IT2可能促进细胞增殖,高葡萄糖条件下HRMEC的迁移和新生血管形成。