Abstract：
OBJECTIVE: Bariatric surgery improves metabolic health, but the underlying mechanisms are not fully understood. We analyzed the effects of two types of bariatric surgery, sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB), on the plasma metabolome and lipidome.
METHODS: We characterized the plasma metabolome (1268 metabolites) and lipidome (953 lipids) pre-operatively and at 3 and 12 months post-operatively in 104 obese adults who were previously recruited to a prospective cohort of bariatric surgery. The metabolomic and lipidomic responses to bariatric surgery over time were analyzed using multivariable linear mixed-effects models.
RESULTS: There were significant changes in multiple metabolites and lipids, including rapid early changes in amino acid and peptide metabolites, including decreases in branched-chain amino acids (BCAAs), aromatic AAs, alanine and aspartate, and increases in glycine, serine, arginine and citrulline. There were also significant decreases in many triglyceride species, with increases in phosphatidylcholines and phosphatidylethanolamines. There were significant changes in metabolites related to energy metabolism that were apparent only after 12 months. We observed differences by bariatric surgery type in the changes in a small number of primary and secondary bile acids, including glycohyocholate and glyco-beta-muricholate.
CONCLUSIONS: Our findings highlight the comprehensive changes in metabolites and lipids that occur over the 12 months following bariatric surgery. While both SG and RYGB caused profound changes in the metabolome and lipidome, RYGB was characterized by greater increases in bile acids following surgery.
METHODS: We characterized the plasma metabolome (1268 metabolites) and lipidome (953 lipids) pre-operatively and at 3 and 12 months post-operatively in 104 obese adults who were previously recruited to a prospective cohort of bariatric surgery. The metabolomic and lipidomic responses to bariatric surgery over time were analyzed using multivariable linear mixed-effects models.
RESULTS: There were significant changes in multiple metabolites and lipids, including rapid early changes in amino acid and peptide metabolites, including decreases in branched-chain amino acids (BCAAs), aromatic AAs, alanine and aspartate, and increases in glycine, serine, arginine and citrulline. There were also significant decreases in many triglyceride species, with increases in phosphatidylcholines and phosphatidylethanolamines. There were significant changes in metabolites related to energy metabolism that were apparent only after 12 months. We observed differences by bariatric surgery type in the changes in a small number of primary and secondary bile acids, including glycohyocholate and glyco-beta-muricholate.
CONCLUSIONS: Our findings highlight the comprehensive changes in metabolites and lipids that occur over the 12 months following bariatric surgery. While both SG and RYGB caused profound changes in the metabolome and lipidome, RYGB was characterized by greater increases in bile acids following surgery.
摘要：
目的：减肥手术改善代谢健康，但是潜在的机制还没有完全理解。我们分析了两种减肥手术的效果，袖状胃切除术（SG）和Roux-en-Y胃旁路术（RYGB），血浆代谢组和脂质组。
方法：我们在术前以及术后3个月和12个月时，对104名肥胖成年人进行了血浆代谢组（1268种代谢物）和脂质（953脂质）的表征，这些人先前被招募到减肥手术的前瞻性队列中。使用多变量线性混合效应模型分析了减肥手术随时间的代谢组学和脂质组学反应。
结果：多种代谢产物和脂质有显著变化，包括氨基酸和肽代谢物的快速早期变化，包括支链氨基酸（BCAAs）的减少，芳香AAs，丙氨酸和天冬氨酸，甘氨酸的增加，丝氨酸,精氨酸和瓜氨酸。许多甘油三酯种类也显著减少，随着磷脂酰胆碱和磷脂酰乙醇胺的增加。与能量代谢相关的代谢物有显著变化，仅在12个月后才明显。我们观察到减肥手术类型的差异在少量的初级和次级胆汁酸的变化，包括糖胆酸盐和糖胞醇盐。
结论：我们的发现强调了减重手术后12个月内代谢产物和脂质的全面变化。虽然SG和RYGB都引起了代谢组和脂质组的深刻变化，RYGB的特征是手术后胆汁酸增加更多。
方法：我们在术前以及术后3个月和12个月时，对104名肥胖成年人进行了血浆代谢组（1268种代谢物）和脂质（953脂质）的表征，这些人先前被招募到减肥手术的前瞻性队列中。使用多变量线性混合效应模型分析了减肥手术随时间的代谢组学和脂质组学反应。
结果：多种代谢产物和脂质有显著变化，包括氨基酸和肽代谢物的快速早期变化，包括支链氨基酸（BCAAs）的减少，芳香AAs，丙氨酸和天冬氨酸，甘氨酸的增加，丝氨酸,精氨酸和瓜氨酸。许多甘油三酯种类也显著减少，随着磷脂酰胆碱和磷脂酰乙醇胺的增加。与能量代谢相关的代谢物有显著变化，仅在12个月后才明显。我们观察到减肥手术类型的差异在少量的初级和次级胆汁酸的变化，包括糖胆酸盐和糖胞醇盐。
结论：我们的发现强调了减重手术后12个月内代谢产物和脂质的全面变化。虽然SG和RYGB都引起了代谢组和脂质组的深刻变化，RYGB的特征是手术后胆汁酸增加更多。
