PDF(Pubmed)
Abstract：
Eczema is a common skin disease associated with inflammation. Interleukin (IL)-24 is crucial in the pathogenesis of inflammatory diseases like eczema. The study objective was the assessment of IL-24 serum levels and its gene polymorphisms in eczematic Iraqi patients. This retrospective case-control study involved 145 participants, divided into 82 patients with eczema and 63 healthy controls. An enzyme-linked immunosorbent assay measured serum IL-24, while polymerase chain reaction and Sanger DNA sequencing were used for genotype analysis. Serum IL-24 level was significantly higher (P value < .001) in patients compared to controls (41.6 [interquartile range (IQR): 28.9-53.6] vs 9.8 [IQR: 0.8-19.6] pg/mL, respectively). DNA sequence illustrated 2 SNPs with polymorphic frequencies (rs1150256 G/A and rs3093425 del/ins). The first SNP (rs1150256 G/A) showed 3 genotypes (GG, AA, and G/A), while the second SNP (rs3093425) showed 3 genotypes (-/G del/Ins, G Ins/Ins, and - del/del). The subsequent investigation revealed the presence of the following findings within the DNA sequence of the PCR amplified region (329bp). In the control group, all participants had GG/G (wild type) genotype/allele for the rs1150256 SNP, while in eczematic patients, 24.4% GG, 50% GA, and 25.6% AA. For the second SNP genotype (rs3093425 del/ins), the genotype frequencies in patients vs control were (24.4% vs 84.1%, 50.0% vs 11.1%, and 25.6% vs 4.8; Del/Del, Del/Ins, and Ins/Ins, respectively). The presence of Ins compared to Del increased the risk of eczema by 8.91 (4.66-17.03); OR (95% CI). In conclusion, IL-24 is a good predictor of eczema and A-allele carrier for rs1150256 SNP, and insertion-allele carrier for rs3093425 SNP is associated with elevated serum IL-24 and higher risk of eczema.
摘要：
湿疹是与炎症相关的常见皮肤病。白细胞介素（IL）-24在湿疹等炎症性疾病的发病机理中至关重要。研究目的是评估伊拉克患者的IL-24血清水平及其基因多态性。这项回顾性病例对照研究涉及145名参与者，分为82例湿疹患者和63例健康对照者。酶联免疫吸附测定法测量了血清IL-24，而聚合酶链反应和SangerDNA测序用于基因型分析。与对照组相比，患者的血清IL-24水平显着升高（P值<.001）（41.6[四分位距（IQR）：28.9-53.6]vs9.8[IQR：0.8-19.6]pg/mL，分别)。DNA序列显示了2个具有多态性频率的SNP（rs1150256G/A和rs3093425del/ins）。第一个SNP（rs1150256G/A）显示3个基因型（GG，AA,和G/A），而第二个SNP(rs3093425)显示3种基因型(-/Gdel/Ins，GIns/Ins,和-del/del)。随后的研究揭示了在PCR扩增区域(329bp)的DNA序列中存在以下发现。在对照组中，所有参与者都有rs1150256SNP的GG/G（野生型）基因型/等位基因，而在湿疹患者中，24.4%GG,50%GA,和25.6%AA。对于第二个SNP基因型(rs3093425del/ins)，患者与对照组的基因型频率为（24.4%vs84.1%，50.0%vs11.1%，25.6%对4.8；德尔/德尔，Del/Ins,和Ins/Ins，分别)。与Del相比，Ins的存在使湿疹的风险增加了8.91（4.66-17.03）;OR（95％CI）。总之,IL-24是湿疹和rs1150256SNP的A等位基因携带者的良好预测因子，rs3093425SNP的插入等位基因携带者与血清IL-24升高和湿疹风险升高相关。
