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Abstract：
This study aimed to elucidate the influence of various culture medium components, including carbon sources, nitrogen sources, inorganic salts, suspension agents, and temperature, on the mycelial growth characteristics of Phallus dongsun. Employing single-factor experiments and response surface methodology within glass Petri dishes, the research identified that carrot powder, soybean powder, and ZnSO4 notably enhanced the proliferation of aerial mycelium, significantly augmenting the growth rate of P. dongsun mycelium. The resultant mycelium was observed to be dense, robust, and fluffy in texture. In particular, ZnSO4 markedly accelerated the mycelium growth rate. Furthermore, xanthan gum was found to effectively modulate the medium\'s viscosity, ensuring a stable suspension and facilitating nutrient equilibrium. The optimal cultivation temperature was determined to be 25°C, with mycelial growth ceasing below 5°C and mycelium perishing at temperatures exceeding 35°C. The optimal medium composition was established as follows: wheat starch 5 g/l, carrot powder 5 g/l, soybean powder 7.50 g/l, glucose 10 g/l, ZnSO4 0.71 g/l, NH4Cl 0.68 g/l, xanthan gum 0.5 g/l, and agar 20 g/l. Under these optimized conditions, the mycelium of P. dongsun exhibited a rapid growth rate (1.04 ± 0.14 mm/day), characterized by a thick, dense, and well-developed structure. This investigation provides a theoretical foundation for the conservation, strain selection, and breeding of P. dongsun.
摘要：
本研究旨在阐明各种培养基成分的影响,包括碳源，氮源，无机盐，悬浮剂，和温度，对冬日阴茎菌丝生长特性的研究。在玻璃培养皿中采用单因素实验和响应面方法，研究发现胡萝卜粉,大豆粉,ZnSO4显著增强了气生菌丝体的增殖，显着提高了东孙菌丝体的生长速度。观察到所得菌丝体致密，健壮,质地蓬松。特别是,ZnSO4显着加速了菌丝体的生长速率。此外，黄原胶被发现有效地调节介质的粘度，确保稳定的悬浮和促进营养平衡。最佳培养温度确定为25°C，菌丝体生长在5°C以下停止，菌丝体在超过35°C的温度下腐烂。最佳培养基组成如下：小麦淀粉5g/l，胡萝卜粉5克/升，大豆粉7.50g/l，葡萄糖10克/升，ZnSO40.71g/l，NH4Cl0.68g/l，黄原胶0.5g/l，和琼脂20克/升。在这些优化条件下，东孙的菌丝体表现出快速的生长速率(1.04±0.14毫米/天)，特点是厚厚的，密集,和发达的结构。这一调查为保护提供了理论基础,应变选择,和东森的繁殖。
