Abstract：
The ultrasonic-assisted deep eutectic solvent method was used to extract the polysaccharides of Pericarpium Citri Reticulatae (PCRP), and the ultrasound-assisted DES extraction process was optimized by Box-Behnken response surface test using the extraction rate of the PCRP as an index; the in vitro activities of purified the PCRP(PCRPs-1) were investigated by determining the scavenging rate of DPPH• and ABTS•+ as well as by enzyme inhibition assay. The monosaccharide composition was analyzed by HPLC. The best process conditions for response surface optimization were a material-liquid ratio of 1:37 g/mL, water content of 44%, time of 89 min, and power of 320 W. The polysaccharide extraction rate was measured to be 5.41%, which was well optimized when compared with that of the ordinary aqueous extraction method of 3.92%. By α-glucosidase and α-amylase inhibition activity test, it showed that the PCRPs-1 had hypoglycemic activity. The DPPH radical scavenging activity test and ABTS + scavenging activity test indicated that the PCRPs-1 had good biological activity. Analysis of the monosaccharide fractions showed that the PCRPs-1 consisted of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose, with molar ratios of 1:39.24:4.41:8.91:7.83:86.00:1.02:9.17. The activity studies showed that PCRPs-1 possessed certain hypoglycaemic and antioxidant activities.
摘要：
采用超声波辅助低共熔溶剂法提取陈皮多糖,以PCRP的提取率为指标，通过Box-Behnken响应面试验优化了超声辅助DES的提取工艺；通过测定DPPH•和ABTS•+的清除率以及酶抑制试验研究了纯化的PCRP（PCRPs-1）的体外活性。通过HPLC分析单糖组成。响应面优化的最佳工艺条件为料液比1:37g/mL,水含量为44%，时间89分钟，功率为320W，多糖提取率为5.41%，与3.92％的普通水提法相比，得到了很好的优化。通过α-葡萄糖苷酶和α-淀粉酶抑制活性试验,这表明PCRPs-1具有降血糖活性。DPPH自由基清除活性和ABTS+清除活性试验表明PCRPs-1具有良好的生物活性。对单糖部分的分析表明PCRPs-1由甘露糖组成，鼠李糖,葡萄糖醛酸，半乳糖醛酸，葡萄糖，半乳糖,木糖,和阿拉伯糖,摩尔比为1:39.24:4.41:8.91:7.83:86.00:1.02:9.17。活性研究表明，PCRPs-1具有一定的降血糖和抗氧化活性。
