Abstract：
Studying human skin biology can aid in comprehending the pathophysiology of skin diseases and developing novel cell-based therapies, including tissue engineering approaches. This chapter provides a comprehensive guide of methods to determine human skin samples from the perspective of their cellular compositions. We describe as useful technique the histological analysis of tissue sections. We further illustrate the biological characterization of isolated and cultured basal and suprabasal interfollicular keratinocytes by cell sorting, cytospin immunostaining, colony forming efficiency, and long-term dermo-epidermal organotypic cultures.
摘要：
研究人类皮肤生物学可以帮助理解皮肤病的病理生理学和开发新的基于细胞的疗法，包括组织工程方法。本章提供了从细胞组成的角度确定人类皮肤样品的方法的全面指南。我们将组织切片的组织学分析描述为有用的技术。我们通过细胞分选进一步说明了分离和培养的基底和基底上毛囊间角质形成细胞的生物学特征，细胞自旋免疫染色，菌落形成效率，和长期的真皮-表皮器官型培养。
