Abstract：
Golden pompano is an important aquaculture product in the coastal regions of southern China, which is highly dependent on insulin-like growth factor (IGF) for various biological processes. The cDNAs of ToIGF1, ToIGF2, and ToIGF3 are 1718 bp, 1658 bp, and 2272 bp in length, respectively, with corresponding amino acid sequences of 185 aa, 215 aa, and 194 aa. These sequences consist of 5 parts, including the signal peptide, the B domain, the C domain, the A domain, the D domain, and the E domain, which are also found in other species. While ToIGF1 has no SSR polymorphism, ToIGF2 and ToIGF3 have 3 and 1 SSR polymorphism sites, respectively. In terms of tissue expression, ToIGF1 is predominantly expressed in the liver, ToIGF2 shows its highest expression in the gills, and ToIGF3 also shows its highest expression in the gills, but no expression in the liver and spleen. These tissue distribution results suggest that ToIGFs are not only present in growth-related tissues such as the brain, muscle, and liver, but also in reproductive tissues, tissues that regulate osmotic pressure, and tissues related to food intake. This observation is consistent with other bony fish species and highlights the extensive biological functions of ToIGFs that need to be further explored and exploited. In addition, the expression levels of ToIGFs were found to be different in the different dietary groups, including the pelleted food group, the frozen squid group, and the frozen fish group. In the pelleted diet group, ToIGF1 and ToIGF2 were highly expressed in the liver and intestinal tissues, followed by the frozen fish group. These results suggest that the type of diet can affect the body\'s energy metabolism by influencing tissue expression of growth-related genes, which in turn affects individual growth.
摘要：
金帕诺是我国南方沿海地区重要的水产养殖产品,在各种生物过程中高度依赖于胰岛素样生长因子（IGF）。ToIGF1、ToIGF2和ToIGF3的cDNA为1718bp,1658bp,长度为2272bp，分别,相应的氨基酸序列为185个氨基酸，215aa,194aa。这些序列由5个部分组成，包括信号肽，B域,C域,A域,D域,和E域，也存在于其他物种中。虽然ToIGF1没有SSR多态性，ToIGF2和ToIGF3有3个和1个SSR多态性位点，分别。在组织表达方面，ToIGF1主要在肝脏中表达，ToIGF2在ill中表达最高，和ToIGF3也显示了它的最高表达，但在肝脏和脾脏中没有表达。这些组织分布结果表明，ToIGFs不仅存在于与生长有关的组织中，例如大脑，肌肉，还有肝脏,而且在生殖组织中，调节渗透压的组织，和与食物摄入有关的组织。这一观察结果与其他硬骨鱼物种一致，并强调了ToIGFs的广泛生物学功能，需要进一步探索和利用。此外,在不同的膳食组中，ToIGFs的表达水平不同，包括颗粒状食物组，冷冻鱿鱼组，还有冷冻鱼群.在颗粒饮食组中，ToIGF1和ToIGF2在肝脏和肠组织中高表达，其次是冷冻鱼组。这些结果表明，饮食类型可以通过影响生长相关基因的组织表达来影响身体的能量代谢，进而影响个体的成长。
