PDF(Pubmed)
Abstract：
Staphylococcus aureus forms biofilms consisting of cells embedded in a matrix made of proteins, polysaccharides, lipids, and extracellular DNA (eDNA). Biofilm-associated infections are difficult to treat and can promote antibiotic resistance, resulting in negative healthcare outcomes. eDNA within the matrix contributes to the stability, growth, and immune-evasive properties of S. aureus biofilms. eDNA is released by autolysis, which is mediated by murein hydrolases that access the cell wall via membrane pores formed by holin-like proteins. The eDNA content of S. aureus biofilms varies among individual strains and is influenced by environmental conditions, including the presence of antibiotics. eDNA plays an important role in biofilm development and structure by acting as an electrostatic net that facilitates protein-cell and cell-cell interactions. Because of eDNA\'s structural importance in biofilms and its ubiquitous presence among S. aureus isolates, it is a potential target for therapeutics. Treatment of biofilms with DNase can eradicate or drastically reduce them in size. Additionally, antibodies that target DNABII proteins, which bind to and stabilize eDNA, can also disperse biofilms. This review discusses the recent literature on the release, structure, and function of eDNA in S. aureus biofilms, in addition to a discussion of potential avenues for targeting eDNA for biofilm eradication.
摘要：
金黄色葡萄球菌形成生物膜，由嵌入蛋白质基质中的细胞组成，多糖，脂质，和细胞外DNA(eDNA)。生物膜相关感染难以治疗，并且可以促进抗生素耐药性，导致负面的医疗保健结果。基质内的eDNA有助于稳定性，增长,和金黄色葡萄球菌生物膜的免疫规避特性。eDNA通过自溶释放，它是由murein水解酶介导的，该水解酶通过holin样蛋白形成的膜孔进入细胞壁。金黄色葡萄球菌生物膜的eDNA含量在各个菌株之间有所不同，并且受环境条件的影响，包括抗生素的存在。eDNA通过充当促进蛋白质-细胞和细胞-细胞相互作用的静电网在生物膜的发育和结构中起重要作用。由于eDNA在生物膜中的结构重要性及其在金黄色葡萄球菌分离物中的普遍存在，它是治疗的潜在目标。用DNA酶处理生物膜可以根除或急剧减小它们的大小。此外,靶向DNABII蛋白的抗体，结合并稳定eDNA，还可以分散生物膜。这篇评论讨论了有关该版本的最新文献，结构,和DNA在金黄色葡萄球菌生物膜中的功能，除了讨论靶向eDNA用于生物膜根除的潜在途径。
