Abstract：
BACKGROUND: Single-platform flow cytometry technology together with CD45-gating is becoming the method of choice for absolute CD4 T cell enumeration. Immunological assessment of HIV patients by monitoring CD4 can provide valuable information on antiviral treatment response and disease progression.
METHODS: A total of 97 HIV-positive individuals were recruited from 2 hospitals in Tripoli, Libya, and 14 healthy blood donors. The HIV-infected individuals were classified by CD4+ count into HIV-positive (>200 cells/µL) or AIDS (≤200 cells/µL) groups. CD4+ and CD8+ cell counts were determined and compared among the groups and with similar published data.
RESULTS: The mean ± SD CD4+ cell counts were 1106 ± 442.8 cells/µL in healthy individuals, 460 ± 219.7 cells/µL in the HIV-positive group, and 78 ± 64.3 cells/µL in the AIDS group. The mean ± SD CD4+/CD8+ ratio was 1.6 ± 0.58, 0.4 ± 0.22, and 0.1 ± 0.1, respectively. CD4+ counts in Libyan healthy adults might be higher than those reported in several studies in other regions, whereas CD4+ counts in Libyan AIDS patients seem lower.
CONCLUSIONS: Reference values for T lymphocyte counts in Libyan healthy individuals should be investigated more extensively, and the reasons why Libyan AIDS patients seem to have such lower CD4+ counts should be examined.
METHODS: A total of 97 HIV-positive individuals were recruited from 2 hospitals in Tripoli, Libya, and 14 healthy blood donors. The HIV-infected individuals were classified by CD4+ count into HIV-positive (>200 cells/µL) or AIDS (≤200 cells/µL) groups. CD4+ and CD8+ cell counts were determined and compared among the groups and with similar published data.
RESULTS: The mean ± SD CD4+ cell counts were 1106 ± 442.8 cells/µL in healthy individuals, 460 ± 219.7 cells/µL in the HIV-positive group, and 78 ± 64.3 cells/µL in the AIDS group. The mean ± SD CD4+/CD8+ ratio was 1.6 ± 0.58, 0.4 ± 0.22, and 0.1 ± 0.1, respectively. CD4+ counts in Libyan healthy adults might be higher than those reported in several studies in other regions, whereas CD4+ counts in Libyan AIDS patients seem lower.
CONCLUSIONS: Reference values for T lymphocyte counts in Libyan healthy individuals should be investigated more extensively, and the reasons why Libyan AIDS patients seem to have such lower CD4+ counts should be examined.
摘要：
背景：单平台流式细胞技术与CD45门控一起正在成为绝对CD4T细胞计数的首选方法。通过监测CD4对HIV患者进行免疫学评估可以为抗病毒治疗反应和疾病进展提供有价值的信息。
方法：从的黎波里的2家医院招募了97名HIV阳性者，利比亚,14名健康的献血者.通过CD4+计数将HIV感染的个体分为HIV阳性(>200细胞/μL)或AIDS(≤200细胞/μL)组。确定CD4+和CD8+细胞计数并在组间进行比较，并与类似的已发表数据进行比较。
结果：健康个体的平均±SDCD4+细胞计数为1106±442.8细胞/μL，HIV阳性组的460±219.7细胞/微升，和艾滋病组78±64.3细胞/μL。平均±SDCD4/CD8比率分别为1.6±0.58、0.4±0.22和0.1±0.1。利比亚健康成年人的CD4+计数可能高于其他地区的几项研究报告。而利比亚艾滋病患者的CD4+计数似乎较低。
结论：应更广泛地研究利比亚健康个体的T淋巴细胞计数参考值，和原因，为什么利比亚艾滋病患者似乎有如此低的CD4+计数应该检查。
方法：从的黎波里的2家医院招募了97名HIV阳性者，利比亚,14名健康的献血者.通过CD4+计数将HIV感染的个体分为HIV阳性(>200细胞/μL)或AIDS(≤200细胞/μL)组。确定CD4+和CD8+细胞计数并在组间进行比较，并与类似的已发表数据进行比较。
结果：健康个体的平均±SDCD4+细胞计数为1106±442.8细胞/μL，HIV阳性组的460±219.7细胞/微升，和艾滋病组78±64.3细胞/μL。平均±SDCD4/CD8比率分别为1.6±0.58、0.4±0.22和0.1±0.1。利比亚健康成年人的CD4+计数可能高于其他地区的几项研究报告。而利比亚艾滋病患者的CD4+计数似乎较低。
结论：应更广泛地研究利比亚健康个体的T淋巴细胞计数参考值，和原因，为什么利比亚艾滋病患者似乎有如此低的CD4+计数应该检查。
