{Reference Type}: Journal Article
{Title}: Absolute CD4 count and percentage values among Libyan patients with HIV by single-platform flow cytometry.
{Author}: Lamami Y;Abulayha AM;Altabal S;Elbasir M;Elbnnani AS;Aghil L;Ebrahim F;Elzagheid A;
{Journal}: Lab Med
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jun 21
{Factor}: 1.865
{DOI}: 10.1093/labmed/lmae046
{Abstract}: <B>BACKGROUND: </B>Single-platform flow cytometry technology together with CD45-gating is becoming the method of choice for absolute CD4 T cell enumeration. Immunological assessment of HIV patients by monitoring CD4 can provide valuable information on antiviral treatment response and disease progression.<BR><B>METHODS: </B>A total of 97 HIV-positive individuals were recruited from 2 hospitals in Tripoli, Libya, and 14 healthy blood donors. The HIV-infected individuals were classified by CD4+ count into HIV-positive (>200 cells/µL) or AIDS (≤200 cells/µL) groups. CD4+ and CD8+ cell counts were determined and compared among the groups and with similar published data.<BR><B>RESULTS: </B>The mean ± SD CD4+ cell counts were 1106 ± 442.8 cells/µL in healthy individuals, 460 ± 219.7 cells/µL in the HIV-positive group, and 78 ± 64.3 cells/µL in the AIDS group. The mean ± SD CD4+/CD8+ ratio was 1.6 ± 0.58, 0.4 ± 0.22, and 0.1 ± 0.1, respectively. CD4+ counts in Libyan healthy adults might be higher than those reported in several studies in other regions, whereas CD4+ counts in Libyan AIDS patients seem lower.<BR><B>CONCLUSIONS: </B>Reference values for T lymphocyte counts in Libyan healthy individuals should be investigated more extensively, and the reasons why Libyan AIDS patients seem to have such lower CD4+ counts should be examined.