Abstract：
The main bioavailable phenolics from of Gongju (GJ) and their mechanism for hepato-protection remain unclear. To select the GJ phenolics with high bioavailability, chrysanthemum digestion and Caco-2 cells were used and their hepato-protective potential were examined by using AML-12 cells. The digestive recovery and small intestinal transit rate of the main phenolic compounds ranged from 28.52 to 69.53% and 6.57% ∼ 15.50%, respectively. Among them, chlorogenic acid, 3,5-dicaffeoylquinic acid, and 1,5-dicaffeoylquinic acid, showed higher small intestinal transit rates and digestive recoveries. Furthermore, we found that by increasing intracellular Catalase (CAT) and Superoxide dismutase (SOD) viability and lowering Malondialdehyde (MDA) level (P < 0.05), 3,5-dicaffeoylquinic acid significantly mitigated the oxidative damage of AML-12 liver cells more than the other two phenolics. Our results demonstrated that 3,5-dicaffeoylquninic acid was the primary phenolic compounds in GJ that effectively reduced liver damage, providing a theoretical basis for the development of GJ as a potentially useful resource for hepatoprotective diet.
摘要：
贡菊（GJ）的主要生物可利用酚类物质及其对肝脏保护的机制尚不清楚。为了选择具有高生物利用度的GJ酚类物质，使用菊花消化和Caco-2细胞，并使用AML-12细胞检查其肝脏保护潜力。主要酚类化合物的消化恢复和小肠转运率分别为28.52%~69.53%和6.57%~15.50%,分别。其中,绿原酸,3,5-二咖啡酰基奎尼酸,和1,5-二咖啡酰基奎尼酸，显示出较高的小肠转运率和消化恢复率。此外，我们发现，通过增加细胞内过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活力和降低丙二醛（MDA）水平（P<0.05），3,5-二咖啡酰基奎尼酸比其他两种酚类更能显著减轻AML-12肝细胞的氧化损伤。我们的结果表明，3,5-二咖啡酰基喹啉酸是主要的酚类化合物在GJ，有效地减少肝损伤，为开发GJ作为保肝饮食的潜在有用资源提供了理论依据。
