PDF(Pubmed)
Abstract：
Toxoplasmosis is an important zoonotic disease caused by Toxoplasma gondii that can infect almost all warm-blooded animals worldwide, including humans. The high prevalence of T. gondii infection and its ability to cause serious harm to humans and animals, especially immunodeficient individuals, make it a key public health issue. Accurate diagnostic tools with high sensitivity are needed for controlling T. gondii infection. In the current study, we compared the performance of recombinant SAG2, GRA6, and GRA7 in ELISA for the serological diagnosis of T. gondii infection in cats. We further investigated the antigenicity of recombinant dense granule protein 3 (rGRA3), rGRA5, rGRA8, and rSRS29A expressed in a plant-based, cell-free expression system for detecting antibodies in T. gondii-infected cats. In summary, our data suggest that GRA7 is more sensitive than the other two antigens for the serodiagnosis of T. gondii infection in cats, and GRA3 expressed in the cell-free system is also a priming antigen in serological tests for detecting T. gondii infection in cats.
摘要：
弓形虫病是由弓形虫引起的一种重要的人畜共患疾病，可感染全世界几乎所有的温血动物，包括人类。弓形虫感染的高患病率及其对人和动物造成严重危害的能力,尤其是免疫缺陷个体，让它成为一个关键的公共卫生问题。需要具有高灵敏度的精确诊断工具来控制弓形虫感染。在目前的研究中,我们比较了重组SAG2，GRA6和GRA7在ELISA中对猫弓形虫感染的血清学诊断的性能。我们进一步研究了重组致密颗粒蛋白3（rGRA3）的抗原性，rGRA5，rGRA8和rSRS29A在植物中表达，用于检测弓形虫感染的猫中抗体的无细胞表达系统。总之,我们的数据表明GRA7对猫弓形虫感染的血清诊断比其他两种抗原更敏感，在无细胞系统中表达的GRA3也是用于检测猫弓形虫感染的血清学测试中的引发抗原。
