PDF(Pubmed)
Abstract：
BACKGROUND: Silica nanoparticles (SNPs) have immense potential in biomedical research, particularly in drug delivery and imaging applications, owing to their stability and minimal interactions with biological entities such as tissues or cells.
RESULTS: With synthesized and characterized cyanine-dye-doped fluorescent SNPs (CSNPs) using cyanine 3.5, 5.5, and 7 (Cy3.5, Cy5.5, and Cy7). Through systematic analysis, we discerned variations in the surface charge and fluorescence properties of the nanoparticles contingent on the encapsulated dye-(3-aminopropyl)triethoxysilane conjugate, while their size and shape remained constant. The fluorescence emission spectra exhibited a redshift correlated with increasing dye concentration, which was attributed to cascade energy transfer and self-quenching effects. Additionally, the fluorescence signal intensity showed a linear relationship with the particle concentration, particularly at lower dye equivalents, indicating a robust performance suitable for imaging applications. In vitro assessments revealed negligible cytotoxicity and efficient cellular uptake of the nanoparticles, enabling long-term tracking and imaging. Validation through in vivo imaging in mice underscored the versatility and efficacy of CSNPs, showing single-switching imaging capabilities and linear signal enhancement within subcutaneous tissue environment.
CONCLUSIONS: This study provides valuable insights for designing fluorescence imaging and optimizing nanoparticle-based applications in biomedical research, with potential implications for targeted drug delivery and in vivo imaging of tissue structures and organs.
RESULTS: With synthesized and characterized cyanine-dye-doped fluorescent SNPs (CSNPs) using cyanine 3.5, 5.5, and 7 (Cy3.5, Cy5.5, and Cy7). Through systematic analysis, we discerned variations in the surface charge and fluorescence properties of the nanoparticles contingent on the encapsulated dye-(3-aminopropyl)triethoxysilane conjugate, while their size and shape remained constant. The fluorescence emission spectra exhibited a redshift correlated with increasing dye concentration, which was attributed to cascade energy transfer and self-quenching effects. Additionally, the fluorescence signal intensity showed a linear relationship with the particle concentration, particularly at lower dye equivalents, indicating a robust performance suitable for imaging applications. In vitro assessments revealed negligible cytotoxicity and efficient cellular uptake of the nanoparticles, enabling long-term tracking and imaging. Validation through in vivo imaging in mice underscored the versatility and efficacy of CSNPs, showing single-switching imaging capabilities and linear signal enhancement within subcutaneous tissue environment.
CONCLUSIONS: This study provides valuable insights for designing fluorescence imaging and optimizing nanoparticle-based applications in biomedical research, with potential implications for targeted drug delivery and in vivo imaging of tissue structures and organs.
摘要：
背景：二氧化硅纳米颗粒（SNPs）在生物医学研究中具有巨大的潜力，特别是在药物输送和成像应用中，由于它们的稳定性和与生物实体如组织或细胞的最小相互作用。
结果:使用花青3.5、5.5和7(Cy3.5、Cy5.5和Cy7)合成和表征花青染料掺杂的荧光SNP(CSNP)。通过系统分析,我们辨别变化的表面电荷和荧光性质的纳米粒子取决于封装的染料-（3-氨基丙基）三乙氧基硅烷共轭，而它们的大小和形状保持不变。荧光发射光谱表现出与染料浓度增加相关的红移，这归因于级联能量转移和自猝灭效应。此外,荧光信号强度与颗粒浓度呈线性关系,特别是在较低的染料当量下，表明适用于成像应用的强大性能。体外评估显示，纳米颗粒的细胞毒性和有效的细胞摄取可忽略不计，实现长期跟踪和成像。通过小鼠体内成像进行的验证强调了CSNP的多功能性和功效，在皮下组织环境中显示单切换成像能力和线性信号增强。
结论：这项研究为设计荧光成像和优化生物医学研究中基于纳米粒子的应用提供了有价值的见解，对靶向药物递送和组织结构和器官的体内成像具有潜在的影响。
结果:使用花青3.5、5.5和7(Cy3.5、Cy5.5和Cy7)合成和表征花青染料掺杂的荧光SNP(CSNP)。通过系统分析,我们辨别变化的表面电荷和荧光性质的纳米粒子取决于封装的染料-（3-氨基丙基）三乙氧基硅烷共轭，而它们的大小和形状保持不变。荧光发射光谱表现出与染料浓度增加相关的红移，这归因于级联能量转移和自猝灭效应。此外,荧光信号强度与颗粒浓度呈线性关系,特别是在较低的染料当量下，表明适用于成像应用的强大性能。体外评估显示，纳米颗粒的细胞毒性和有效的细胞摄取可忽略不计，实现长期跟踪和成像。通过小鼠体内成像进行的验证强调了CSNP的多功能性和功效，在皮下组织环境中显示单切换成像能力和线性信号增强。
结论：这项研究为设计荧光成像和优化生物医学研究中基于纳米粒子的应用提供了有价值的见解，对靶向药物递送和组织结构和器官的体内成像具有潜在的影响。
