Abstract：
In this work, the selenylation Codonopsis pilosula polysaccharide (Se-CPPS) were synthesized using an optimized microwave-assisted method. Then, physicochemical properties, including molecular weight, particle size, valence state of selenium, antioxidant capacity, release mechanism of selenium under gastrointestinal conditions, as well as their effects on HT-29 cell viability were comprehensively investigated. The results demonstrated that Se-CPPS with the highest selenium content (21.71 mg/g) was synthesized using 0.8% nitric acid concentration under microwave conditions of 90 min at 70 °C. FTIR and XPS analysis revealed that Se was bound to the polysaccharide chain in the form of O-Se-O and O-H···Se, with a valence state of either 0 or +4. In vitro investigations on antioxidant activity and selenium release capacity indicated that selenization not only enhanced the antioxidant activity of CPPS but also endowed Se-CPPS with robust selenium release capability in simulated gastric digestion. The effects of Se-CPPS on HT-29 cells was further investigated by CCK-8 method. The results showed that the selenide modification effectively reduced the toxicity of Na2SeO3 and enhanced the viability of CPPS. The findings of this study offer valuable methodological guidance for the synthesis of Se-polysaccharides with superior functional properties.
摘要：
在这项工作中,使用优化的微波辅助方法合成了硒化党参多糖（Se-CPPS）。然后，物理化学性质，包括分子量，颗粒大小,硒的价态，抗氧化能力，硒在胃肠道条件下的释放机制，以及它们对HT-29细胞活力的影响进行了全面研究。结果表明，硒含量最高（21.71mg/g）的Se-CPPS是使用0.8%硝酸浓度在70°C下90分钟的微波条件下合成的。FTIR和XPS分析表明，Se以O-Se-O和O-H···Se的形式与多糖链结合，价态为0或+4。对抗氧化活性和硒释放能力的体外研究表明，硒化不仅增强了CPPS的抗氧化活性，而且使Se-CPPS在模拟胃消化中具有强大的硒释放能力。通过CCK-8方法进一步研究Se-CPPS对HT-29细胞的影响。结果表明，硒化物改性有效降低了Na2SeO3的毒性，增强了CPPS的活力。这项研究的结果为合成具有优异功能特性的硒多糖提供了有价值的方法学指导。
