Abstract：
The widespread adoption of Poly(3-hydroxybutyrate) (PHB) encounters challenges due to its higher production costs compared to conventional plastics. To overcome this obstacle, this study investigates the use of low-cost raw materials and optimized production methods. Specifically, food processing byproducts such as corn germ and corn bran were utilized as solid substrates through solid-state fermentation, enriched with molasses and cheese whey. Employing the One Factor at a Time technique, we examined the effects of substrate composition, temperature, initial substrate moisture, molasses, and cheese whey on PHB production at the flask scale. Subsequently, experiments were conducted at the bioreactor scale to evaluate the influence of aeration. In flask-scale experiments, the highest PHB yield, reaching 4.1 (g/kg Initial Dry Weight Substrate) (IDWS) after 72 hours, was achieved using a substrate comprising a 1:1 mass ratio of corn germ to corn bran supplemented with 20 % (v/w) cheese whey. Furthermore, PHB production in a 0.5-L packed-bed bioreactor yielded a maximum of 8.4 (g/kg IDWS), indicating a more than 100 % increase in yield after 72 hours, with optimal results achieved at an aeration rate of 0.5 l/(kg IDWS. h).
摘要：
聚(3-羟基丁酸酯)(PHB)的广泛采用由于其与常规塑料相比更高的生产成本而面临挑战。为了克服这个障碍，这项研究调查了低成本原材料的使用和优化的生产方法。具体来说,通过固态发酵，利用玉米胚芽和玉米麸皮等食品加工副产品作为固体基质，富含糖蜜和奶酪乳清。一次采用一个因素的技术，我们检查了底物组成的影响，温度,初始基质水分，以烧瓶规模生产PHB的糖蜜和奶酪乳清。随后，在生物反应器规模进行实验，以评估曝气的影响。在烧瓶规模的实验中，最高的PHB产量，72小时后达到4.1（g/kg初始干重底物）（IDWS），使用包含1:1质量比的补充有20%(v/w)奶酪乳清的玉米胚芽与玉米麸皮的底物来实现。此外，在0.5升填充床生物反应器中的PHB产量最大为8.4（g/kgIDWS），表明72小时后产量增加超过100%，在曝气率为0.5l/(kgIDWS。h).
