Abstract：
BACKGROUND: In Brazilian popular medicine, Lippia alba leaves are used in teas to treat pain and inflammatory diseases.
OBJECTIVE: to evaluate the chemical composition, antinociceptive, and anti-inflammatory activities of Lippia alba essential oil and its major compound geraniol.
METHODS: Lippia alba leaves were collected in Pará state, Brazil. The leaf essential oil was obtained using a modified Clevenger-type extractor. Then, the oil was analyzed by GC and GC-MS analyses. To evaluate the toxicity of LaEO and geraniol, the doses of 50, 300, and 2000 mg/kg were used in a mouse model. For antinociception tests, abdominal contortion, hot plate, and formalin tests were used; all groups were treated with LaEO and geraniol at doses of 25, 50, and 100 mg/kg; and to evaluate inflammation using the ear edema model.
RESULTS: The constituents identified in the highest content were oxygenated monoterpenes: geraniol (37.5%), geranial (6.7%) and neral (3.8%). The animals treated with LaEO and geraniol demonstrated atypical behaviors with aspects of lethargy and drowsiness, characteristics of animals in a state of sedation; the relative weights showed no significant difference compared to the controls. In the abdominal contortion test, LaEO at 25 mg/kg, 50 mg/kg doses, and 100 mg/kg reduced the number of contortions, representing a percentage reduction of 84.64%, 81.23%, and 66.21% respectively. In the hot plate test, LaEO and geraniol increased the latency time at doses of 25, 50, and 100 mg/kg in all test periods; there was no statistical difference between LaEO and geraniol. In the first phase of the formalin test, only doses of 25 mg/kg and 100 mg/kg of LaEO showed significant activity, reducing the latency time by 53.40% and 58.90%. LaEO at doses of 25 mg/kg and 100 mg/kg reduced the size of the edema, demonstrating an anti-inflammatory activity of 59.38% (25 mg/kg) and 50% (100 mg/kg).
CONCLUSIONS: Lippia alba essential oil and geraniol showed central/peripheral analgesic and anti-inflammatory potential and can be used as an alternative or complementary treatment to conventional drugs. More studies are needed to evaluate its action mechanisms and its analgesic effects.
OBJECTIVE: to evaluate the chemical composition, antinociceptive, and anti-inflammatory activities of Lippia alba essential oil and its major compound geraniol.
METHODS: Lippia alba leaves were collected in Pará state, Brazil. The leaf essential oil was obtained using a modified Clevenger-type extractor. Then, the oil was analyzed by GC and GC-MS analyses. To evaluate the toxicity of LaEO and geraniol, the doses of 50, 300, and 2000 mg/kg were used in a mouse model. For antinociception tests, abdominal contortion, hot plate, and formalin tests were used; all groups were treated with LaEO and geraniol at doses of 25, 50, and 100 mg/kg; and to evaluate inflammation using the ear edema model.
RESULTS: The constituents identified in the highest content were oxygenated monoterpenes: geraniol (37.5%), geranial (6.7%) and neral (3.8%). The animals treated with LaEO and geraniol demonstrated atypical behaviors with aspects of lethargy and drowsiness, characteristics of animals in a state of sedation; the relative weights showed no significant difference compared to the controls. In the abdominal contortion test, LaEO at 25 mg/kg, 50 mg/kg doses, and 100 mg/kg reduced the number of contortions, representing a percentage reduction of 84.64%, 81.23%, and 66.21% respectively. In the hot plate test, LaEO and geraniol increased the latency time at doses of 25, 50, and 100 mg/kg in all test periods; there was no statistical difference between LaEO and geraniol. In the first phase of the formalin test, only doses of 25 mg/kg and 100 mg/kg of LaEO showed significant activity, reducing the latency time by 53.40% and 58.90%. LaEO at doses of 25 mg/kg and 100 mg/kg reduced the size of the edema, demonstrating an anti-inflammatory activity of 59.38% (25 mg/kg) and 50% (100 mg/kg).
CONCLUSIONS: Lippia alba essential oil and geraniol showed central/peripheral analgesic and anti-inflammatory potential and can be used as an alternative or complementary treatment to conventional drugs. More studies are needed to evaluate its action mechanisms and its analgesic effects.
摘要：
背景：在巴西流行医学中，Lippiaalba叶用于茶治疗疼痛和炎症性疾病。
目的：评价化学成分，抗伤害性,和抗炎活性羊皮脂精油及其主要化合物香叶醇。
方法：在帕拉州收集Lippiaalba叶，巴西。使用改良的Clevenger型提取器获得叶精油。然后，通过GC和GC-MS分析对油进行分析。为了评估LaEO和香叶醇的毒性，在小鼠模型中使用50,300和2000mg/kg的剂量。对于镇痛试验，腹部扭曲,热板,和福尔马林试验;所有组均接受LaEO和香叶醇治疗,剂量为25,50和100mg/kg;并使用耳水肿模型评估炎症.
结果：含量最高的成分是含氧单萜：香叶醇（37.5％），geranial(6.7%)和neral(3.8%)。用LaEO和香叶醇治疗的动物表现出非典型行为，表现出嗜睡和嗜睡。处于镇静状态的动物的特征；相对重量与对照组相比没有显着差异。在腹部扭曲试验中，LaEO在25mg/kg，50mg/kg剂量，100毫克/千克减少扭曲的数量，减少了84.64%，81.23%，和分别为66.21%。在热板测试中，在所有测试期间，LaEO和香叶醇以25、50和100mg/kg的剂量增加了潜伏期；LaEO和香叶醇之间没有统计学差异。在福尔马林测试的第一阶段，只有25mg/kg和100mg/kg剂量的LaEO显示出显著的活性，减少了53.40%和58.90%的延迟时间。25mg/kg和100mg/kg剂量的LaEO减少了水肿的大小，显示59.38%(25mg/kg)和50%(100mg/kg)的抗炎活性。
结论：唇皮精油和香叶醇具有中枢/外周镇痛和抗炎潜力，可作为常规药物的替代或补充治疗。需要更多的研究来评估其作用机制及其镇痛作用。
目的：评价化学成分，抗伤害性,和抗炎活性羊皮脂精油及其主要化合物香叶醇。
方法：在帕拉州收集Lippiaalba叶，巴西。使用改良的Clevenger型提取器获得叶精油。然后，通过GC和GC-MS分析对油进行分析。为了评估LaEO和香叶醇的毒性，在小鼠模型中使用50,300和2000mg/kg的剂量。对于镇痛试验，腹部扭曲,热板,和福尔马林试验;所有组均接受LaEO和香叶醇治疗,剂量为25,50和100mg/kg;并使用耳水肿模型评估炎症.
结果：含量最高的成分是含氧单萜：香叶醇（37.5％），geranial(6.7%)和neral(3.8%)。用LaEO和香叶醇治疗的动物表现出非典型行为，表现出嗜睡和嗜睡。处于镇静状态的动物的特征；相对重量与对照组相比没有显着差异。在腹部扭曲试验中，LaEO在25mg/kg，50mg/kg剂量，100毫克/千克减少扭曲的数量，减少了84.64%，81.23%，和分别为66.21%。在热板测试中，在所有测试期间，LaEO和香叶醇以25、50和100mg/kg的剂量增加了潜伏期；LaEO和香叶醇之间没有统计学差异。在福尔马林测试的第一阶段，只有25mg/kg和100mg/kg剂量的LaEO显示出显著的活性，减少了53.40%和58.90%的延迟时间。25mg/kg和100mg/kg剂量的LaEO减少了水肿的大小，显示59.38%(25mg/kg)和50%(100mg/kg)的抗炎活性。
结论：唇皮精油和香叶醇具有中枢/外周镇痛和抗炎潜力，可作为常规药物的替代或补充治疗。需要更多的研究来评估其作用机制及其镇痛作用。
