PDF(Pubmed)
Abstract：
BACKGROUND: valproic acid (VPA), an epigenetic drug, has potential for the treatment of neoplasms. Its effects on the healing of the peritoneal-musculo-aponeurotic plane (PMA) of the abdominal wall are studied.
METHODS: sixty Wistar rats were allocated into two groups: experimental (VPA) and control (0.9% sodium chloride), treated daily, starting three days before the intervention and until euthanasia. Under anesthesia, a median laparotomy was performed and repaired with two synthetic layers. Assessments took place 3, 7 and 14 days after surgery. The integrity of the wounds, the quality of the inflammatory reaction, the intensity of the leukocyte infiltrate, collagen synthesis, the intensity of angiogenesis and the presence of myofibroblasts were studied.
RESULTS: there was dehiscence of the PMA plane in 11 of the 30 animals (p=0.001) in the experimental group. There was no difference in the quality and intensity of the inflammatory reaction. Immunohistochemistry revealed, in the experimental group, less collagen I (p3=0.003, p7=0.013 and p14=0.001) and more collagen III (p3=0.003, p7=0.013 and p14= 0.001). Collagen evaluated by Sirus Supra Red F3BA showed, in the experimental group, less collagen at all three times (p<0.001) with less collagen I and collagen III (p<0.001). A lower number of vessels was found on the 3rd day (p<0.001) and on the 7th day (p=0.001) and did not affect the number of myofibroblasts.
CONCLUSIONS: VPA showed dehiscence of the PMA plane, with less deposition of total collagen and collagen I, less angiogenic activity, without interfering with the number of myofibroblasts.
METHODS: sixty Wistar rats were allocated into two groups: experimental (VPA) and control (0.9% sodium chloride), treated daily, starting three days before the intervention and until euthanasia. Under anesthesia, a median laparotomy was performed and repaired with two synthetic layers. Assessments took place 3, 7 and 14 days after surgery. The integrity of the wounds, the quality of the inflammatory reaction, the intensity of the leukocyte infiltrate, collagen synthesis, the intensity of angiogenesis and the presence of myofibroblasts were studied.
RESULTS: there was dehiscence of the PMA plane in 11 of the 30 animals (p=0.001) in the experimental group. There was no difference in the quality and intensity of the inflammatory reaction. Immunohistochemistry revealed, in the experimental group, less collagen I (p3=0.003, p7=0.013 and p14=0.001) and more collagen III (p3=0.003, p7=0.013 and p14= 0.001). Collagen evaluated by Sirus Supra Red F3BA showed, in the experimental group, less collagen at all three times (p<0.001) with less collagen I and collagen III (p<0.001). A lower number of vessels was found on the 3rd day (p<0.001) and on the 7th day (p=0.001) and did not affect the number of myofibroblasts.
CONCLUSIONS: VPA showed dehiscence of the PMA plane, with less deposition of total collagen and collagen I, less angiogenic activity, without interfering with the number of myofibroblasts.
摘要：
背景：丙戊酸（VPA），一种表观遗传药物，具有治疗肿瘤的潜力。研究了其对腹壁腹膜-肌肉-肌腱膜平面（PMA）愈合的影响。
方法：将60只Wistar大鼠分为两组：实验（VPA）和对照组（0.9％氯化钠），每日治疗,从干预前三天开始，直到安乐死。在麻醉下,我们进行了正中剖腹手术,并用两个合成层进行了修复.在手术后3、7和14天进行评估。伤口的完整性，炎症反应的质量，白细胞浸润的强度，胶原蛋白合成，研究了血管生成的强度和肌成纤维细胞的存在。
结果:实验组30只动物中有11只(p=0.001)PMA平面开裂。炎症反应的质量和强度没有差异。免疫组织化学显示，在实验组中，I型胶原较少（p3=0.003，p7=0.013和p14=0.001），而III型胶原较多（p3=0.003，p7=0.013和p14=0.001）。通过SirusSupraRedF3BA评估的胶原蛋白显示，在实验组中，所有三次的胶原蛋白都较少（p<0.001），胶原蛋白I和胶原蛋白III较少（p<0.001）。在第3天（p<0.001）和第7天（p=0.001）发现较低数量的血管，并且不影响肌成纤维细胞的数量。
结论：VPA显示PMA平面开裂，总胶原蛋白和胶原蛋白I的沉积较少，血管生成活性较低，而不干扰肌成纤维细胞的数量。
方法：将60只Wistar大鼠分为两组：实验（VPA）和对照组（0.9％氯化钠），每日治疗,从干预前三天开始，直到安乐死。在麻醉下,我们进行了正中剖腹手术,并用两个合成层进行了修复.在手术后3、7和14天进行评估。伤口的完整性，炎症反应的质量，白细胞浸润的强度，胶原蛋白合成，研究了血管生成的强度和肌成纤维细胞的存在。
结果:实验组30只动物中有11只(p=0.001)PMA平面开裂。炎症反应的质量和强度没有差异。免疫组织化学显示，在实验组中，I型胶原较少（p3=0.003，p7=0.013和p14=0.001），而III型胶原较多（p3=0.003，p7=0.013和p14=0.001）。通过SirusSupraRedF3BA评估的胶原蛋白显示，在实验组中，所有三次的胶原蛋白都较少（p<0.001），胶原蛋白I和胶原蛋白III较少（p<0.001）。在第3天（p<0.001）和第7天（p=0.001）发现较低数量的血管，并且不影响肌成纤维细胞的数量。
结论：VPA显示PMA平面开裂，总胶原蛋白和胶原蛋白I的沉积较少，血管生成活性较低，而不干扰肌成纤维细胞的数量。
