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Abstract：
BACKGROUND: Bersama abyssinica Fresen is a plant that is used in folk medicine for the treatment of mastitis and other infectious diseases.
OBJECTIVE: The antibacterial activity of methanol crude extract of plant was evaluated against three common bacterial pathogens, including Gram positive (Staphylococcus aureus) and Gram negative (Escherichia coli and Pseudomonas aeruginosa).
METHODS: The antibacterial activities and minimum inhibitory concentration of B. abyssinica crude extracts were evaluated using agar-well diffusion and broth dilution methods according to the National Committee for Clinical Laboratory Standards (NCCLS).
RESULTS: A significant difference in the antibacterial activity of crude extracts was observed among different levels of concentration against tested isolates. A higher mean inhibition zone diameter was recorded in E. coli (29.2 ± 1.5 mm), followed by S. aureus (27.8 ± 1.1 mm) and P. aeruginosa (18.0 ± 0.7 mm) at a concentration of 100 mg/mL. The antibacterial activity of crude plant extract at 100 mg/mL was comparable with that of a standard antibiotic (27.6 ± 2.6) against S. aureus and E. coli isolates. The findings indicated that bacterial growth inhibition increased as the concentration of the crude extracts increased. E. coli and S. aureus isolates showed significantly higher susceptibilities to crude extracts than P. aeruginosa at all concentrations. The minimum inhibitory concentrations of extracts against S. aureus, E. coli and P. aeruginosa isolates were 0.78 mg/mL, 1.56 mg/mL and 1.56 mg/mL, respectively.
CONCLUSIONS: All tested pathogenic bacterial species were susceptible to plant leaf extract and broad-spectrum activity against Gram-positive and Gram-negative bacteria. The study recommends further fractionation of the B. abyssinica plant that contributes to its antibacterial activity and understands the mode of action of this plant against bacteria and other microbes.
OBJECTIVE: The antibacterial activity of methanol crude extract of plant was evaluated against three common bacterial pathogens, including Gram positive (Staphylococcus aureus) and Gram negative (Escherichia coli and Pseudomonas aeruginosa).
METHODS: The antibacterial activities and minimum inhibitory concentration of B. abyssinica crude extracts were evaluated using agar-well diffusion and broth dilution methods according to the National Committee for Clinical Laboratory Standards (NCCLS).
RESULTS: A significant difference in the antibacterial activity of crude extracts was observed among different levels of concentration against tested isolates. A higher mean inhibition zone diameter was recorded in E. coli (29.2 ± 1.5 mm), followed by S. aureus (27.8 ± 1.1 mm) and P. aeruginosa (18.0 ± 0.7 mm) at a concentration of 100 mg/mL. The antibacterial activity of crude plant extract at 100 mg/mL was comparable with that of a standard antibiotic (27.6 ± 2.6) against S. aureus and E. coli isolates. The findings indicated that bacterial growth inhibition increased as the concentration of the crude extracts increased. E. coli and S. aureus isolates showed significantly higher susceptibilities to crude extracts than P. aeruginosa at all concentrations. The minimum inhibitory concentrations of extracts against S. aureus, E. coli and P. aeruginosa isolates were 0.78 mg/mL, 1.56 mg/mL and 1.56 mg/mL, respectively.
CONCLUSIONS: All tested pathogenic bacterial species were susceptible to plant leaf extract and broad-spectrum activity against Gram-positive and Gram-negative bacteria. The study recommends further fractionation of the B. abyssinica plant that contributes to its antibacterial activity and understands the mode of action of this plant against bacteria and other microbes.
摘要：
背景：百萨马苦艾香是一种在民间医学中用于治疗乳腺炎和其他传染病的植物。
目的：评价植物甲醇粗提物对3种常见病原菌的抑菌活性。包括革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（大肠杆菌和铜绿假单胞菌）。
方法：根据国家临床实验室标准委员会（NCCLS），使用琼脂扩散和肉汤稀释方法评估了深渊芽孢杆菌粗提物的抗菌活性和最低抑菌浓度。
结果：在不同浓度水平下，观察到粗提物的抗菌活性存在显着差异。在大肠杆菌中记录到更高的平均抑制区直径(29.2±1.5mm),其次是金黄色葡萄球菌（27.8±1.1毫米）和铜绿假单胞菌（18.0±0.7毫米），浓度为100毫克/毫升。100mg/mL的粗植物提取物对金黄色葡萄球菌和大肠杆菌分离株的抗菌活性与标准抗生素（27.6±2.6）相当。结果表明，细菌生长抑制作用随着粗提物浓度的增加而增加。大肠杆菌和金黄色葡萄球菌分离株在所有浓度下对粗提物的敏感性均显著高于铜绿假单胞菌。提取物对金黄色葡萄球菌的最低抑制浓度，大肠杆菌和铜绿假单胞菌的分离率为0.78mg/mL，1.56mg/mL和1.56mg/mL，分别。
结论：所有受试病原菌对植物叶提取物敏感，对革兰氏阳性菌和革兰氏阴性菌具有广谱活性。该研究建议进一步分级分离深渊芽孢杆菌植物，这有助于其抗菌活性，并了解该植物对细菌和其他微生物的作用方式。
目的：评价植物甲醇粗提物对3种常见病原菌的抑菌活性。包括革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（大肠杆菌和铜绿假单胞菌）。
方法：根据国家临床实验室标准委员会（NCCLS），使用琼脂扩散和肉汤稀释方法评估了深渊芽孢杆菌粗提物的抗菌活性和最低抑菌浓度。
结果：在不同浓度水平下，观察到粗提物的抗菌活性存在显着差异。在大肠杆菌中记录到更高的平均抑制区直径(29.2±1.5mm),其次是金黄色葡萄球菌（27.8±1.1毫米）和铜绿假单胞菌（18.0±0.7毫米），浓度为100毫克/毫升。100mg/mL的粗植物提取物对金黄色葡萄球菌和大肠杆菌分离株的抗菌活性与标准抗生素（27.6±2.6）相当。结果表明，细菌生长抑制作用随着粗提物浓度的增加而增加。大肠杆菌和金黄色葡萄球菌分离株在所有浓度下对粗提物的敏感性均显著高于铜绿假单胞菌。提取物对金黄色葡萄球菌的最低抑制浓度，大肠杆菌和铜绿假单胞菌的分离率为0.78mg/mL，1.56mg/mL和1.56mg/mL，分别。
结论：所有受试病原菌对植物叶提取物敏感，对革兰氏阳性菌和革兰氏阴性菌具有广谱活性。该研究建议进一步分级分离深渊芽孢杆菌植物，这有助于其抗菌活性，并了解该植物对细菌和其他微生物的作用方式。
