PDF(Pubmed)
Abstract：
The transcription factors of WRKY genes play essential roles in plant growth, stress responses, and metabolite biosynthesis. Erigeron breviscapus, a traditional Chinese herb, is abundant in flavonoids and has been used for centuries to treat cardiovascular and cerebrovascular diseases. However, the WRKY transcription factors that regulate flavonoid biosynthesis in E. breviscapus remain unknown. In this study, a total of 75 EbWRKY transcription factors were predicted through comprehensive genome-wide characterization of E. breviscapus and the chromosomal localization of each EbWRKY gene was investigated. RNA sequencing revealed transient responses of 74 predicted EbWRKY genes to exogenous abscisic acid (ABA), salicylic acid (SA), and gibberellin 3 (GA3) after 4 h of treatment. In contrast, the expression of key structural genes involved in flavonoid biosynthesis increased after 4 h in GA3 treatment. However, the content of flavonoid metabolites in leaves significantly increased at 12 h. The qRT-PCR results showed that the expression patterns of EbWRKY11, EbWRKY30, EbWRKY31, EbWRKY36, and EbWRKY44 transcription factors exhibited a high degree of similarity to the 11 structural genes involved in flavonoid biosynthesis. Protein-DNA interactions were performed between the key genes involved in scutellarin biosynthesis and candidate WRKYs. The result showed that F7GAT interacts with EbWRKY11, EbWRKY36, and EbWRKY44, while EbF6H has a self-activation function. This study provides comprehensive information on the regulatory control network of flavonoid accumulation mechanisms, offering valuable insights for breeding E. breviscapus varieties with enhanced scutellarin content.
摘要：
WRKY基因的转录因子在植物生长中起着至关重要的作用,应激反应，和代谢产物的生物合成。灯盏细辛,一种传统的中草药，富含类黄酮，几个世纪以来一直被用来治疗心脑血管疾病。然而,在灯盏细辛中调节类黄酮生物合成的WRKY转录因子仍然未知。在这项研究中,通过对灯盏细辛菌进行全面的全基因组鉴定,共预测了75个EbWRKY转录因子,并研究了每个EbWRKY基因的染色体定位.RNA测序揭示了74个预测的EbWRKY基因对外源脱落酸（ABA）的瞬时反应，水杨酸(SA),和赤霉素3（GA3）治疗4小时后。相比之下,GA3处理4h后，参与类黄酮生物合成的关键结构基因的表达增加。然而,在12h时，叶片中类黄酮代谢产物的含量显着增加。qRT-PCR结果表明，EbWRKY11，EbWRKY30，EbWRKY31，EbWRKY36和EbWRKY44转录因子的表达模式与11个参与类黄酮生物合成的结构基因具有高度相似性。在参与灯盏乙素生物合成的关键基因和候选WRKYs之间进行蛋白质-DNA相互作用。结果表明，F7GAT与EbWRKY11，EbWRKY36和EbWRKY44相互作用，而EbF6H具有自激活功能。本研究为类黄酮积累机制的调控网络提供了全面的信息，为培育灯盏细辛含量增加的灯盏细辛品种提供有价值的见解。
