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Abstract：
UNASSIGNED: We aimed to distinguish the different Chinese medicine (CM) syndromes of acquired immune deficiency syndrome (AIDS) patients at the proteomics level.
UNASSIGNED: We collected AIDS patients diagnosed with different CM syndromes from Weishi County, Kaifeng City, Henan Province, China, including Qi-deficiency syndrome (named QD group) and dampness-heat syndrome (named DH group). Healthy people were collected as controls from Weishi County, Kaifeng city, Henan Province, China. The plasma from three groups were labeled with ITRAQ, LC/MC was used for protein quantitative analysis. Finally, sequence search and cluster analysis were performed.
UNASSIGNED: Overall, 27 different proteins were found. Three proteins were up-regulated and 2 proteins down-regulated in the QD group, 11 proteins up-regulated and 13 proteins down-regulated in the DH group. Compared with DH group, there were 7 different proteins in QD group, among which 5 proteins were down-regulated and 2 proteins were up-regulated. When the target protein of DH group was up-regulated, the protein of HC group was down-regulated correspondingly.
UNASSIGNED: The significance analysis and clustering of protein results showed that DH group was significantly different from QD group and HC group at the protein level (P<0.05). However, the QD group could not be effectively distinguished from the HC group. AAT, PF4, C-reactive protein and c4bp may be used as potential biomarkers in DH group. Mass spectrometry based on feature selection can be used to classify different CM syndromes.
UNASSIGNED: We collected AIDS patients diagnosed with different CM syndromes from Weishi County, Kaifeng City, Henan Province, China, including Qi-deficiency syndrome (named QD group) and dampness-heat syndrome (named DH group). Healthy people were collected as controls from Weishi County, Kaifeng city, Henan Province, China. The plasma from three groups were labeled with ITRAQ, LC/MC was used for protein quantitative analysis. Finally, sequence search and cluster analysis were performed.
UNASSIGNED: Overall, 27 different proteins were found. Three proteins were up-regulated and 2 proteins down-regulated in the QD group, 11 proteins up-regulated and 13 proteins down-regulated in the DH group. Compared with DH group, there were 7 different proteins in QD group, among which 5 proteins were down-regulated and 2 proteins were up-regulated. When the target protein of DH group was up-regulated, the protein of HC group was down-regulated correspondingly.
UNASSIGNED: The significance analysis and clustering of protein results showed that DH group was significantly different from QD group and HC group at the protein level (P<0.05). However, the QD group could not be effectively distinguished from the HC group. AAT, PF4, C-reactive protein and c4bp may be used as potential biomarkers in DH group. Mass spectrometry based on feature selection can be used to classify different CM syndromes.
摘要：
■我们旨在在蛋白质组学水平上区分获得性免疫缺陷综合征（AIDS）患者的不同中医（CM）综合征。
■我们收集了渭氏县诊断为不同CM综合征的艾滋病患者，开封市,河南省,中国,包括气虚证（QD组）和湿热证（DH组）。从渭氏县收集健康人群作为对照，开封市,河南省,中国。三组的血浆用ITRAQ标记，LC/MC用于蛋白质定量分析。最后,进行序列搜索和聚类分析。
■总的来说，发现了27种不同的蛋白质。QD组有3种蛋白上调，2种蛋白下调，DH组中11种蛋白质上调,13种蛋白质下调。与DH组相比,QD组有7种不同的蛋白质，其中5种蛋白下调，2种蛋白上调。当DH组的靶蛋白上调时，HC组蛋白表达下调。
■蛋白质结果的显著性分析和聚类表明，在蛋白质水平上，DH组与QD组和HC组差异有统计学意义（P<0.05）。然而,QD组与HC组无法有效区分.AAT,PF4、C反应蛋白和c4bp可作为DH组潜在的生物标志物。基于特征选择的质谱可用于对不同的CM综合征进行分类。
■我们收集了渭氏县诊断为不同CM综合征的艾滋病患者，开封市,河南省,中国,包括气虚证（QD组）和湿热证（DH组）。从渭氏县收集健康人群作为对照，开封市,河南省,中国。三组的血浆用ITRAQ标记，LC/MC用于蛋白质定量分析。最后,进行序列搜索和聚类分析。
■总的来说，发现了27种不同的蛋白质。QD组有3种蛋白上调，2种蛋白下调，DH组中11种蛋白质上调,13种蛋白质下调。与DH组相比,QD组有7种不同的蛋白质，其中5种蛋白下调，2种蛋白上调。当DH组的靶蛋白上调时，HC组蛋白表达下调。
■蛋白质结果的显著性分析和聚类表明，在蛋白质水平上，DH组与QD组和HC组差异有统计学意义（P<0.05）。然而,QD组与HC组无法有效区分.AAT,PF4、C反应蛋白和c4bp可作为DH组潜在的生物标志物。基于特征选择的质谱可用于对不同的CM综合征进行分类。
