Abstract：
Increasing bone diseases and anomalies significantly challenge bone regeneration, necessitating the development of innovative implantable devices for effective healing. This study explores the potential of 3D-printed calcium phosphate (CaP) scaffolds functionalized with natural medicine to address this issue. Specifically, quercetin and vitamin D3 (QVD) encapsulated solid lipid nanoparticles (QVD-SLNs) are incorporated into the scaffold to enhance bone regeneration. The melt emulsification method is utilized to achieve high drug encapsulation efficiency (~98%) and controlled biphasic release kinetics. The process-structure-property performance of these systems allows more controlled release while maintaining healthy cell-material interactions. The functionalized scaffolds show ~1.3- and ~-1.6-fold increase in osteoblast cell proliferation and differentiation, respectively, as compared with the control. The treated scaffold demonstrates a reduction in osteoclastic activity as compared with the control. The QVD-SLN-loaded scaffolds show ~4.2-fold in vitro chemopreventive potential against osteosarcoma cells. Bacterial assessment with both Staphylococcus aureus and Pseudomonas aeruginosa shows a significant reduction in bacterial colony growth over the treated scaffold. These findings summarize that the release of QVD-SLNs through a 3D-printed CaP scaffold can treat various bone-related disorders for low or non-load-bearing applications.
摘要：
增加的骨骼疾病和异常显着挑战骨再生，需要开发创新的可植入设备以实现有效的治疗。这项研究探讨了用天然药物功能化的3D打印磷酸钙（CaP）支架来解决这个问题的潜力。具体来说,槲皮素和维生素D3（QVD）包封的固体脂质纳米颗粒（QVD-SLN）被掺入支架中以增强骨再生。熔融乳化方法用于实现高药物包封率(〜98%)和受控的双相释放动力学。这些系统的过程-结构-性质性能允许更受控的释放,同时保持健康的细胞-材料相互作用。功能化的支架显示成骨细胞增殖和分化增加~1.3-和~-1.6倍，分别,与对照组相比。与对照相比,经处理的支架显示破骨细胞活性降低。负载QVD-SLN的支架显示出约4.2倍的体外针对骨肉瘤细胞的化学预防潜力。对金黄色葡萄球菌和铜绿假单胞菌的细菌评估显示,在处理的支架上细菌菌落生长显著减少。这些发现总结了通过3D打印的CaP支架释放QVD-SLN可以治疗各种骨相关疾病，用于低负荷或非负荷应用。
