PDF(Pubmed)
Abstract：
Sedum telephium is a succulent plant used in traditional medicine, particularly in Italy, for its efficacy in treating localized inflammation such as burns, warts, and wounds. Fresh leaves or freshly obtained derivatives are directly applied to the injuries for these purposes. However, challenges such as the lack of microbiologically controlled materials and product standardization prompted the exploration of more controlled biotechnological alternatives, utilizing in vitro plant cell cultures of S. telephium. In the present study, we used HPLC-DAD analysis to reveal a characteristic flavonol profile in juices from in vivo leaves and in vitro materials mainly characterized by several kaempferol and quercetin derivatives. The leaf juice exhibited the highest content in total flavonol and kaempferol derivatives, whereas juice from callus grown in medium with hormones and callus suspensions showed elevated levels of quercetin derivatives. The in vitro anti-inflammatory and wound-healing assays evidenced the great potential of callus and suspension cultures in dampening inflammation and fostering wound closure, suggesting quercetin may have a pivotal role in biological activities.
摘要：
景天是一种用于传统医学的肉质植物，尤其是在意大利,因为它在治疗局部炎症如烧伤方面的功效，疣,和伤口。出于这些目的,将新鲜的叶子或新鲜获得的衍生物直接施用于损伤。然而,缺乏微生物控制材料和产品标准化等挑战促使人们探索更多受控的生物技术替代品，利用心电链球菌的体外植物细胞培养。在本研究中,我们使用HPLC-DAD分析揭示了体内叶片和体外材料中主要特征为几种山奈酚和槲皮素衍生物的果汁中的黄酮醇特征。叶汁中总黄酮和山奈酚衍生物含量最高，而在含有激素和愈伤组织悬浮液的培养基中生长的愈伤组织的汁液显示槲皮素衍生物水平升高。体外抗炎和伤口愈合试验证明了愈伤组织和悬浮培养物在抑制炎症和促进伤口闭合方面的巨大潜力。这表明槲皮素可能在生物活性中起关键作用。
