PDF(Pubmed)
Abstract：
Polyphenols are plant secondary metabolites that function mostly as a general stress-induced protective mechanism. Polyphenols have also gained interest due to their beneficial properties for human health. Strawberry leaves represent an agro-industrial waste material with relevant bioactive polyphenol content, which could be incorporated into circular economy strategies. However, due to the low quantities of polyphenols in plants, their production needs to be improved for cost-effective applications. The objective of this research was to compare polyphenol production in strawberry (Fragaria × ananassa cv. Festival) leaves in plants grown in greenhouse conditions and plants grown in vitro, using three possible elicitor treatments (UV irradiation, cold exposure, and cysteine). General vegetative effects were morphologically evaluated, and specific polyphenolic compounds were quantified by UHPLC-DAD-MS/MS. Gallic acid was the most abundant polyphenol found in the leaves, both in vivo and in vitro. The results showed higher amounts and faster accumulation of polyphenols in the in vitro regenerated plants, highlighting the relevance of in vitro tissue culture strategies for producing compounds such as polyphenols in this species and cultivar.
摘要：
多酚是植物次生代谢产物，主要作为一般胁迫诱导的保护机制发挥作用。多酚也因其对人类健康的有益性质而受到关注。草莓叶代表具有相关生物活性多酚含量的农业工业废料，可以纳入循环经济战略。然而,由于植物中的多酚含量低，他们的生产需要提高成本效益的应用。这项研究的目的是比较草莓中多酚的产量（Fragaria×ananassacv。节日）在温室条件下生长的植物和在体外生长的植物中的叶子，使用三种可能的激发子处理(紫外线照射，冷暴露,和半胱氨酸）。一般的植物性影响进行形态学评估，和特定的多酚化合物通过UHPLC-DAD-MS/MS进行定量。没食子酸是叶子中最丰富的多酚，体内和体外。结果表明，在体外再生植物中，多酚的含量更高，积累更快。强调了在该物种和品种中生产多酚等化合物的体外组织培养策略的相关性。
