PDF(Pubmed)
Abstract：
Vaginally administered postbiotics derived from Lactobacillus were recently demonstrated to be effective in alleviating bacterial vaginosis and increasing pregnancy rates. However, their potential effect on sperm quality has not been well investigated. This controlled in vitro study aimed to assess the dose- and time-dependent effects of postbiotics derived from Lactobacillus rhamnosus PB01 (DSM 14870) on sperm quality parameters. The experiment was conducted in vitro to eliminate potential confounding factors from the female reproductive tract and vaginal microbiota. Sperm samples from 18 healthy donors were subjected to analysis using Computer-Aided Sperm Analysis (CASA) in various concentrations of postbiotics and control mediums at baseline, 60 min, and 90 min of incubation. Results indicated that lower postbiotic concentration (PB5) did not adversely affect sperm motility, kinematic parameters, sperm DNA fragmentation, and normal morphology at any time. However, concentrations exceeding 15% demonstrated a reduction in progressively motile sperm and a negative correlation with non-progressively motile sperm at all time points. These findings underscore the importance of balancing postbiotic dosage to preserve sperm motility while realizing the postbiotics\' vaginal health benefits. Further research is warranted to understand the underlying mechanisms and refine practical applications in reproductive health.
摘要：
最近证明，阴道给药的乳酸菌后元可有效缓解细菌性阴道病并提高妊娠率。然而,它们对精子质量的潜在影响尚未得到很好的研究。这项受控的体外研究旨在评估鼠李糖乳杆菌PB01（DSM14870）对精子质量参数的剂量和时间依赖性影响。该实验在体外进行以消除来自女性生殖道和阴道微生物群的潜在混杂因素。使用计算机辅助精子分析（CASA）对来自18名健康供体的精子样本进行分析，在基线时使用各种浓度的postbiotics和对照培养基，60分钟,和90分钟的孵育。结果表明，较低的后生物浓度（PB5）不会对精子运动产生不利影响，运动学参数，精子DNA碎片，和正常的形态在任何时候。然而,超过15％的浓度表明，在所有时间点，逐渐活动的精子都减少，并且与非逐渐活动的精子呈负相关。这些发现强调了平衡后生物剂量以保持精子活力，同时实现后生物对阴道健康有益的重要性。有必要进行进一步的研究，以了解潜在的机制并完善生殖健康的实际应用。
