PDF(Pubmed)
Abstract：
WRKYs are a multigenic family of transcription factors that are plant-specific and involved in the regulation of plant development and various stress response processes. However, the evolution of WRKY genes is not fully understood. This family has also been incompletely studied in grapevine, and WRKY genes have been named with different numbers in different studies, leading to great confusion. In this work, 62 Vitis vinifera WRKY genes were identified based on six genomes of different cultivars. All WRKY genes were numbered according to their chromosomal location, and a complete revision of the numbering was performed. Amino acid variability between different cultivars was assessed for the first time and was greater than 5% for some WRKYs. According to the gene structure, all WRKYs could be divided into two groups: more exons/long length and fewer exons/short length. For the first time, some chimeric WRKY genes were found in grapevine, which may play a specific role in the regulation of different processes: VvWRKY17 (an N-terminal signal peptide region followed by a non-cytoplasmic domain) and VvWRKY61 (Frigida-like domain). Five phylogenetic clades A-E were revealed and correlated with the WRKY groups (I, II, III). The evolution of WRKY was studied, and we proposed a WRKY evolution model where there were two dynamic phases of complexity and simplification in the evolution of WRKY.
摘要：
WRKYs是一类多基因转录因子家族，具有植物特异性，参与植物发育和各种胁迫反应过程的调控。然而,WRKY基因的进化尚不完全清楚。这个家族在葡萄藤中也没有得到充分的研究，WRKY基因在不同的研究中被命名为不同的数字，导致巨大的混乱。在这项工作中,根据不同品种的六个基因组鉴定了62个葡萄WRKY基因。所有WRKY基因根据其染色体位置进行编号，并对编号进行了完整的修订。首次评估了不同品种之间的氨基酸变异性，对于某些WRKY，该变异性大于5％。根据基因结构,所有WRKY可分为两组：更多的外显子/长和更少的外显子/短长度。第一次,在葡萄中发现了一些嵌合的WRKY基因，它们可能在不同过程的调节中起特定作用：VvWRKY17（一个N末端信号肽区，随后是非细胞质结构域）和VvWRKY61（Frigida样结构域）。揭示了五个系统进化枝A-E，并与WRKY组相关（I，II,III).研究了WRKY的演变，提出了一个WRKY演化模型，其中WRKY的演化存在复杂性和简化两个动态阶段。
