PDF(Pubmed)
Abstract：
Currently, there is an increase in the aging of the population, which represents a risk factor for many diseases, including sarcopenia. Sarcopenia involves progressive loss of mass, strength, and function of the skeletal muscle. Some mechanisms include alterations in muscle structure, reduced regenerative capacity, oxidative stress, mitochondrial dysfunction, and inflammation. The zebrafish has emerged as a new model for studying skeletal muscle aging because of its numerous advantages, including histological and molecular similarity to human skeletal muscle. In this study, we used fish of 2, 10, 30, and 60 months of age. The older fish showed a higher frailty index with a value of 0.250 ± 0.000 because of reduced locomotor activity and alterations in biometric measurements. We observed changes in muscle structure with a decreased number of myocytes (0.031 myocytes/μm2 ± 0.004 at 60 months) and an increase in collagen with aging up to 15% ± 1.639 in the 60-month group, corresponding to alterations in the synthesis, degradation, and differentiation pathways. These changes were accompanied by mitochondrial alterations, such as a nearly 50% reduction in the number of intermyofibrillar mitochondria, 100% mitochondrial damage, and reduced mitochondrial dynamics. Overall, we demonstrated a similarity in the aging processes of muscle aging between zebrafish and mammals.
摘要：
目前,人口老龄化加剧，这代表了许多疾病的危险因素，包括肌肉减少症.肌肉减少症涉及质量的进行性丧失,力量,和骨骼肌的功能。一些机制包括肌肉结构的改变，再生能力降低，氧化应激，线粒体功能障碍，和炎症。斑马鱼已成为研究骨骼肌衰老的新模型，因为它具有许多优点，包括与人类骨骼肌的组织学和分子相似性。在这项研究中,我们用的是2、10、30和60个月大的鱼。由于运动活动减少和生物识别测量的改变，较老的鱼表现出更高的虚弱指数，值为0.250±0.000。我们观察到肌肉结构的变化，在60个月组中，肌细胞数量减少（60个月时为0.031个肌细胞/μm2±0.004），胶原蛋白随着年龄增长而增加15％±1.639，对应于合成中的变化，降解，和分化途径。这些变化伴随着线粒体改变，例如肌纤维间线粒体的数量减少了近50%，100%线粒体损伤,减少线粒体动力学。总的来说,我们证明了斑马鱼和哺乳动物在肌肉衰老过程中的相似性。
