PDF(Pubmed)
Abstract：
Glycans of MVs are proposed to be candidates for mediating targeting specificity or at least promoting it. In contrast to exosomes, glycomic studies of MVs are largely absent. We studied the glycoprofile of endothelial cell-derived MVs using 21 plant lectins, and the results show the dominance of oligolactosamines and their α2-6-sialylated forms as N-glycans and low levels of α2-3-sialylated glycans. The low levels of α2-3-sialosides could not be explained by the action of extracellular glycosidases. Additionally, the level of some Man-containing glycans was also decreased in MVs. Spatial masking as the causative relationship between these low level glycans (as glycosphingolipids) by integral proteins or proteoglycans (thus, their lack of interaction with lectins) seems unlikely. The results suggest that integral proteins do not pass randomly into MVs, but instead only some types, differing in terms of their specific glycosylation, are integrated into MVs.
摘要：
提出MV的聚糖是介导靶向特异性或至少促进其的候选物。与外泌体相比，对MV的糖色研究基本上没有。我们使用21种植物凝集素研究了内皮细胞衍生的MV的糖谱，结果显示寡糖胺及其α2-6-唾液酸化形式作为N-聚糖的优势和低水平的α2-3-唾液酸化聚糖。α2-3-唾液酸苷的低水平不能通过细胞外糖苷酶的作用来解释。此外,MVs中一些含Man聚糖的水平也降低。空间掩蔽作为这些低水平聚糖（作为鞘糖脂）之间的致病关系，通过整合蛋白质或蛋白聚糖（因此，他们缺乏与凝集素的互动)似乎不太可能。结果表明，整合蛋白不会随机进入MV，但只有一些类型，在它们的特定糖基化方面有所不同，集成到MV中。
