PDF(Pubmed)
Abstract：
The utility of serum glial fibrillary acidic protein (GFAP) in acute ischemic stroke (AIS) has been extensively studied in recent years. Here, we aimed to assess its potential role as a cargo protein of extracellular vesicles (EVs) secreted by astrocytes (ADEVs) in response to brain ischemia. Plasma samples from eighteen AIS patients at 24 h (D1), 7 days (D7), and one month (M1) post-symptoms onset, and nine age, sex, and cardiovascular risk factor-matched healthy controls were obtained to isolate EVs using the Exoquick ULTRA EV kit. Subsets of presumed ADEVs were identified further by the expression of the glutamate aspartate transporter (GLAST) as a specific marker of astrocytes with the Basic Exo-Flow Capture kit. Western blotting has tested the presence of GFAP in ADEV cargo. Post-stroke ADEV GFAP levels were elevated at D1 and D7 but not M1 compared to controls (p = 0.007, p = 0.019, and p = 0.344, respectively). Significant differences were highlighted in ADEV GFAP content at the three time points studied (n = 12, p = 0.027) and between D1 and M1 (z = 2.65, p = 0.023). A positive correlation was observed between the modified Rankin Scale (mRS) at D7 and ADEV GFAP at D1 (r = 0.58, p = 0.010) and D7 (r = 0.57, p = 0.013), respectively. ADEV GFAP may dynamically reflect changes during the first month post-ischemia. Profiling ADEVs from peripheral blood could provide a new way to assess the central nervous system pathology.
摘要：
近年来，已广泛研究了血清胶质纤维酸性蛋白（GFAP）在急性缺血性中风（AIS）中的用途。这里,我们旨在评估其作为星形胶质细胞(ADEVs)分泌的细胞外囊泡(EVs)的货物蛋白在脑缺血反应中的潜在作用.24小时18例AIS患者的血浆样本（D1），7天（D7），症状发作后一个月（M1），九岁，性别,和心血管危险因素匹配的健康对照使用ExoquickULTRAEV试剂盒分离EV。通过用碱性Exo-Flow捕获试剂盒表达作为星形胶质细胞的特异性标记的谷氨酸天冬氨酸转运蛋白(GLAST)进一步鉴定了推定的ADEVs的亚群。Western印迹测试了ADEV货物中GFAP的存在。与对照组相比，卒中后ADEVGFAP水平在D1和D7时升高，但在M1时则没有升高（分别为p=0.007，p=0.019和p=0.344）。在研究的三个时间点（n=12，p=0.027）以及D1和M1之间（z=2.65，p=0.023），ADEVGFAP含量存在显着差异。D7的改良Rankin量表（mRS）与D1（r=0.58，p=0.010）和D7（r=0.57，p=0.013）的ADEVGFAP之间呈正相关。分别。ADEVGFAP可以动态地反映缺血后第一个月期间的变化。从外周血中分析ADEV可以为评估中枢神经系统病理提供新的方法。
