PDF(Pubmed)
Abstract：
Formin Homology Proteins (Formins) are a highly conserved family of cytoskeletal regulatory proteins that participate in a diverse range of cellular processes. FMNL2 is a member of the Diaphanous-Related Formin sub-group, and previous reports suggest FMNL2\'s role in filopodia assembly, force generation at lamellipodia, subcellular trafficking, cell-cell junction assembly, and focal adhesion formation. How FMNL2 is recruited to these sites of action is not well understood. To shed light on how FMNL2 activity is partitioned between subcellular locations, we used biotin proximity labeling and proteomic analysis to identify an FMNL2 interactome. The interactome identified known and new FMNL2 interacting proteins with functions related to previously described FMNL2 activities. In addition, our interactome predicts a novel connection between FMNL2 and extracellular vesicle assembly. We show directly that FMNL2 protein is present in exosomes.
摘要：
Formin同源蛋白质(Formins)是高度保守的细胞骨架调节蛋白家族,其参与多种细胞过程。FMNL2是与透明相关的Formin子小组的成员，和以前的报道表明FMNL2在丝状体组装中的作用，在薄片上产生力，亚细胞贩运，电池-电池连接组件，和病灶粘连形成。FMNL2如何被招募到这些作用位点尚不清楚。为了阐明FMNL2活动是如何在亚细胞位置之间划分的，我们使用生物素邻近标记和蛋白质组分析来鉴定FMNL2相互作用组。相互作用组鉴定了具有与先前描述的FMNL2活性相关的功能的已知和新的FMNL2相互作用蛋白。此外,我们的相互作用组预测了FMNL2和细胞外囊泡组装之间的新联系。我们直接表明FMNL2蛋白存在于外泌体中。
