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Abstract：
The root system plays a decisive role in the growth and development of plants. The water requirement of a root system depends strongly on the plant species. Potatoes are an important food and vegetable crop grown worldwide, especially under irrigation in arid and semi-arid regions. However, the expected impact of global warming on potato yields calls for an investigation of genes related to root development and drought resistance signaling pathways in potatoes. In this study, we investigated the molecular mechanisms of different drought-tolerant potato root systems in response to drought stress under controlled water conditions, using potato as a model. We analyzed the transcriptome and proteome of the drought-sensitive potato cultivar Atlantic (Atl) and the drought-tolerant cultivar Qingshu 9 (Q9) under normal irrigation (CK) and weekly drought stress (D). The results showed that a total of 14,113 differentially expressed genes (DEGs) and 5596 differentially expressed proteins (DEPs) were identified in the cultivars. A heat map analysis of DEGs and DEPs showed that the same genes and proteins in Atl and Q9 exhibited different expression patterns under drought stress. Weighted gene correlation network analysis (WGCNA) showed that in Atl, Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG)-enriched pathways were related to pyruvate metabolism and glycolysis, as well as cellular signaling and ion transmembrane transporter protein activity. However, GO terms and KEGG-enriched pathways related to phytohormone signaling and the tricarboxylic acid cycle were predominantly enriched in Q9. The present study provides a unique genetic resource to effectively explore the functional genes and uncover the molecular regulatory mechanism of the potato root system in response to drought stress.
摘要：
根系在植物的生长发育中起着决定性的作用。根系的需水量在很大程度上取决于植物物种。马铃薯是一种重要的粮食和蔬菜作物,特别是在干旱和半干旱地区的灌溉下。然而,全球变暖对马铃薯产量的预期影响要求研究与马铃薯根系发育和抗旱信号通路相关的基因。在这项研究中,我们研究了在受控水分条件下不同耐旱马铃薯根系响应干旱胁迫的分子机制，用土豆做模型.我们分析了正常灌溉（CK）和每周干旱胁迫（D）下干旱敏感马铃薯品种大西洋（Atl）和耐旱品种青树9（Q9）的转录组和蛋白质组。结果表明，在品种中总共鉴定出14,113个差异表达基因（DEGs）和5596个差异表达蛋白（DEPs）。对DEGs和DEP的热图分析表明，在干旱胁迫下，Atl和Q9中相同的基因和蛋白质表现出不同的表达模式。加权基因相关网络分析(WGCNA)显示,在Atl,基因本体论（GO）术语和京都基因和基因组百科全书（KEGG）富集途径与丙酮酸代谢和糖酵解有关，以及细胞信号传导和离子跨膜转运蛋白活性。然而,与植物激素信号传导和三羧酸循环相关的GO术语和KEGG富集途径主要在Q9中富集。本研究为有效探索马铃薯根系响应干旱胁迫的功能基因和分子调控机制提供了独特的遗传资源。
