PDF(Pubmed)
Abstract：
Sweet basil (Ocimum basilicum L.) is an important aromatic plant with high edibility and economic value, widely distributed in many regions of the tropics including the south of China. In recent years, environmental problems, especially soil salinization, have seriously restricted the planting and spread of sweet basil. However, the molecular mechanism of the salt stress response in sweet basil is still largely unknown. In this study, seed germination, seedling growth, and chlorophyll synthesis in sweet basil were inhibited under salt stress conditions. Through comparative transcriptome analysis, the gene modules involved in the metabolic processes, oxidative response, phytohormone signaling, cytoskeleton, and photosynthesis were screened out. In addition, the landscape of transcription factors during salt treatment in sweet basil was displayed as well. Moreover, the overexpression of the WRKY transcription factor-encoding gene, ObWRKY16, and the phenylalanine ammonia-lyase-encoding gene, ObPAL2, enhanced the seed germination, seedling growth, and survival rate, respectively, of transgenic Arabidopsis, suggesting that they might be important candidates for the creation of salt-tolerant sweet basil cultivars. Our data enrich the study on salt responses in sweet basil and provide essential gene resources for genetic improvements in sweet basil in the future.
摘要：
甜罗勒(OcimumbasilicumL.)是一种重要的芳香植物,具有很高的食用性和经济价值,广泛分布在包括中国南方在内的许多热带地区。近年来,环境问题,特别是土壤盐渍化，严重限制了甜罗勒的种植和传播。然而,甜罗勒盐应激反应的分子机制尚不清楚。在这项研究中,种子萌发,幼苗生长，在盐胁迫条件下，甜罗勒的叶绿素合成受到抑制。通过比较转录组分析，参与代谢过程的基因模块，氧化反应，植物激素信号，细胞骨架,并筛选出光合作用。此外,还显示了甜罗勒盐处理期间转录因子的景观。此外，WRKY转录因子编码基因的过表达，ObWRKY16和苯丙氨酸解氨酶编码基因，ObPAL2，增强种子萌发，幼苗生长，和存活率，分别,转基因拟南芥，这表明它们可能是创建耐盐甜罗勒品种的重要候选人。我们的数据丰富了甜罗勒中盐反应的研究，并为未来甜罗勒的遗传改良提供了必要的基因资源。
