PDF(Pubmed)
Abstract：
UVA exposure disturbs the metabolism of skin cells, often inducing oxidative stress and inflammation. Therefore, there is a need for bioactive compounds that limit such consequences without causing undesirable side effects. The aim of this study was to analyse in vitro the effects of the phytocannabinoids cannabigerol (CBG) and cannabidiol (CBD), which differ in terms of biological effects. Furthermore, the combined use of both compounds (CBG+CBD) has been analysed in order to increase their effectiveness in human skin fibroblasts and keratinocytes protection against UVA-induced alternation. The results obtained indicate that the effects of CBG and CBD on the redox balance might indeed be enhanced when both phytocannabinoids are applied concurrently. Those effects include a reduction in NOX activity, ROS levels, and a modification of thioredoxin-dependent antioxidant systems. The reduction in the UVA-induced lipid peroxidation and protein modification has been confirmed through lower levels of 4-HNE-protein adducts and protein carbonyl groups as well as through the recovery of collagen expression. Modification of antioxidant signalling (Nrf2/HO-1) through the administration of CBG+CBD has been proven to be associated with reduced proinflammatory signalling (NFκB/TNFα). Differential metabolic responses of keratinocytes and fibroblasts to the effects of the UVA and phytocannabinoids have indicated possible beneficial protective and regenerative effects of the phytocannabinoids, suggesting their possible application for the purpose of limiting the harmful impact of the UVA on skin cells.
摘要：
UVA暴露会干扰皮肤细胞的新陈代谢，经常引起氧化应激和炎症。因此,需要限制这种后果而不引起不希望的副作用的生物活性化合物。这项研究的目的是在体外分析植物大麻素大麻酚（CBG）和大麻二酚（CBD）的作用，在生物学效应方面有所不同。此外，已经分析了两种化合物（CBGCBD）的组合使用，以增加它们在人皮肤成纤维细胞和角质形成细胞中针对UVA诱导的交替的保护作用。获得的结果表明，当同时应用两种植物大麻素时，CBG和CBD对氧化还原平衡的影响可能确实得到增强。这些影响包括降低NOX活性，ROS水平,以及硫氧还蛋白依赖性抗氧化系统的修饰。UVA诱导的脂质过氧化和蛋白质修饰的减少已通过较低水平的4-HNE-蛋白质加合物和蛋白质羰基以及通过胶原蛋白表达的恢复得到证实。通过施用CBG+CBD修饰抗氧化剂信号(Nrf2/HO-1)已被证明与减少的促炎信号(NFκB/TNFα)相关。角质形成细胞和成纤维细胞对UVA和植物大麻素的影响的差异代谢反应表明植物大麻素可能具有有益的保护和再生作用。这表明它们可能用于限制UVA对皮肤细胞的有害影响。
