PDF(Pubmed)
Abstract：
Apis mellifera filamentous virus (AmFV) is a double-stranded DNA virus that infects Apis mellifera bees. To our knowledge, this is the first comprehensive study aiming to detect and analyse the genetic diversity and prevalence of AmFV in Korean honeybee colonies. Phylogenetic analysis based on baculovirus repeat open reading frame-N gene (Bro-N) sequences revealed that AmFV isolates from the Republic of Korea (ROK) fell into two distinct lineages, with genetic origins in Switzerland and China, with nucleotide similarities of 98.3% and 98.2%, respectively. Our prevalence analysis demonstrated a noteworthy infection rate of AmFV in 545 honeybee colonies, reaching 33.09% in 2022 and increasing to 44.90% by 2023. Intriguingly, we also detected AmFV in Varroa destructor mites, highlighting their potential role as vectors and carriers of AmFV. The presence of AmFV was correlated with an increased infection rate of sacbrood virus, deformed wing virus, Lake Sinai virus 2, black queen cell virus, and Nosema ceranae in honeybee colonies. These findings provide valuable insight into the prevalence and potential transmission mechanisms of AmFV in honeybee colonies in the ROK. The results of this study may be instrumental in the effective management of viral infections in honeybee apiaries.
摘要：
蜜蜂丝状病毒（AmFV）是一种感染蜜蜂的双链DNA病毒。据我们所知,这是第一项旨在检测和分析韩国蜜蜂殖民地AmFV遗传多样性和患病率的综合研究。基于杆状病毒重复开放阅读框N基因（Bro-N）序列的系统发育分析显示，来自大韩民国（ROK）的AmFV分离株分为两个不同的谱系，基因起源于瑞士和中国，核苷酸相似性为98.3%和98.2%，分别。我们的患病率分析表明，在545个蜜蜂群体中，AmFV的感染率值得注意。2022年达到33.09%，到2023年提高到44.90%。有趣的是，我们还在瓦螨体内检测到AmFV，强调他们作为AmFV载体和载体的潜在作用。AmFV的存在与囊状病毒感染率的增加有关，畸形的翅膀病毒,西奈湖病毒2号黑皇后细胞病毒,和蜜蜂殖民地的Nosemaceranae。这些发现为AmFV在韩国蜜蜂群落中的流行和潜在传播机制提供了有价值的见解。这项研究的结果可能有助于有效管理蜜蜂的病毒感染。
