Abstract：
Green and white chemistry are vital to revolutionizing the chemical industry through their unparalleled potential to enhance sustainability and efficiency. In this study, nine sustainability tools of both green and white metrics, including green analytical procedure index (GAPI), ComplexGAPI, analytical greenness, analytical greenness metric for sample preparation, Analytical Eco-Scale (ESA), analytical method greenness score, high-performance liquid chromatography- environmental assessment tool (HPLC-EAT), analytical method volume intensity, and blue applicability grade index (BAGI), have been developed for appraising environmental friendliness for both innovative and straightforward mean centering of ratio spectra (MCR) and reversed-phase high-performance liquid chromatography (RP-HPLC) strategies utilized for concurrent analysis and separation of cyclopentolate (CYC) and C12 and C14 homologs of benzalkonium chloride (BNZ) in pure and ophthalmic solution. The mobile phase, formed of buffer phosphate and acetonitrile (35:65, v/v), was adjusted to pH 6.3, and 215-nm UV detection was used. The experimental flow rate was 2.0 mL min-1, and the analytical column was L11 Inertsil Ph-3 (150 mm × 4.6 mm, 5 µm). All sequences were run at 25°C in the column oven. The MCR approach effectively resolved the drug\'s spectral overlapping. CYC and BNZ employed this approach at 227.5 and 220.4 nm, respectively. As part of the HPLC analysis, an isocratic method was employed with phosphate buffer and acetonitrile in the mobile phase at 35:65. A correlation coefficient greater than 0.999 was observed between the calibration curves for the HPLC and MCR methods in the ranges of 20-320 µg mL-1 and 5-30 µg mL-1 for all drugs. The technique yields excellent primary recovery rates, ranging from 97.2% to 100.5%. The recommended approach has been validated according to International Council for Harmonization guidelines.
摘要：
绿色和白色化学通过其无与伦比的潜力来提高可持续性和效率，对化学工业的革命性至关重要。在这项研究中,绿色和白色指标的九种可持续发展工具，包括绿色分析程序指数(GAPI)，ComplexGAPI,分析绿色，样品制备的分析绿色度量，分析生态尺度(ESA)分析方法绿色度评分，高效液相色谱-环境评估工具（HPLC-EAT），分析方法体积强度，和蓝色适用性等级指数(BAGI)，已开发用于评估环境友好性的创新和简单的平均定心比率光谱（MCR）和反相高效液相色谱（RP-HPLC）策略，用于同时分析和分离环戊酸酯（CYC）和C12和C14同系物在纯溶液和眼用溶液中苯扎氯铵（BNZ）。流动相，由缓冲液磷酸盐和乙腈（35:65，v/v）形成，调节至pH6.3,并使用215nm的UV检测。实验流速为2.0mLmin-1，分析柱为L11InertsilPh-3（150mm×4.6mm，5µm)。所有序列在25°C下在柱烘箱中运行。MCR方法有效地解决了药物的光谱重叠。CYC和BNZ在227.5和220.4nm处采用了这种方法，分别。作为HPLC分析的一部分,采用等度法，磷酸盐缓冲液和乙腈在35:65的流动相中。在所有药物的20-320µgmL-1和5-30µgmL-1范围内，HPLC和MCR方法的校准曲线之间的相关系数均大于0.999。该技术产生优异的初级回收率，从97.2%到100.5%不等。建议的方法已根据国际协调理事会准则得到验证。
