Abstract：
The phylum Oomycota contains economically important pathogens of animals and plants, including Saprolegnia parasitica, the causal agent of the fish disease saprolegniasis. Due to intense fish farming and banning of the most effective control measures, saprolegniasis has re-emerged as a major challenge for the aquaculture industry. Oomycete cells are surrounded by a polysaccharide-rich cell wall matrix that, in addition to being essential for cell growth, also functions as a protective \"armor.\" Consequently, the enzymes responsible for cell wall synthesis provide potential targets for disease control. Oomycete cell wall biosynthetic enzymes are predicted to be plasma membrane proteins. To identify these proteins, we applied a quantitative (iTRAQ) mass spectrometry-based proteomics approach to the plasma membrane of the hyphal cells of S. parasitica, providing the first complete plasma membrane proteome of an oomycete species. Of significance is the identification of 65 proteins enriched in detergent-resistant microdomains (DRMs). In silico analysis showed that DRM-enriched proteins are mainly involved in molecular transport and β-1,3-glucan synthesis, potentially contributing to pathogenesis. Moreover, biochemical characterization of the glycosyltransferase activity in these microdomains further supported their role in β-1,3-glucan synthesis. Altogether, the knowledge gained in this study provides a basis for developing disease control measures targeting specific plasma membrane proteins in S. parasitica.IMPORTANCEThe significance of this research lies in its potential to combat saprolegniasis, a detrimental fish disease, which has resurged due to intensive fish farming and regulatory restrictions. By targeting enzymes responsible for cell wall synthesis in Saprolegnia parasitica, this study uncovers potential avenues for disease control. Particularly noteworthy is the identification of several proteins enriched in membrane microdomains, offering insights into molecular mechanisms potentially involved in pathogenesis. Understanding the role of these proteins provides a foundation for developing targeted disease control measures. Overall, this research holds promise for safeguarding the aquaculture industry against the challenges posed by saprolegniasis.
摘要：
卵菌门含有经济上重要的动植物病原体，包括寄生腐殖质,鱼病腐殖质病的病原体。由于密集的鱼类养殖和禁止最有效的控制措施，腐殖质病已重新成为水产养殖业的主要挑战。卵菌细胞被富含多糖的细胞壁基质包围，除了对细胞生长至关重要之外，还可以作为保护性的“盔甲”。\"因此，负责细胞壁合成的酶为疾病控制提供了潜在的靶标。卵菌细胞壁生物合成酶被预测为质膜蛋白。为了鉴定这些蛋白质，我们将定量（iTRAQ）基于质谱的蛋白质组学方法应用于寄生链球菌菌丝细胞的质膜，提供卵菌物种的第一个完整的质膜蛋白质组。重要的是鉴定富含洗涤剂抗性微域(DRMs)的65种蛋白质。计算机分析表明，DRM富集蛋白主要参与分子转运和β-1,3-葡聚糖合成，可能导致发病机制。此外，这些微域中糖基转移酶活性的生化表征进一步支持了它们在β-1,3-葡聚糖合成中的作用。总之,在这项研究中获得的知识为制定针对寄生链球菌的特定质膜蛋白的疾病控制措施提供了基础。重要意义这项研究的意义在于其对抗腐乳质的潜力，一种有害的鱼类疾病，由于集约化的鱼类养殖和监管限制，这种情况已经恢复。通过靶向寄生虫中负责细胞壁合成的酶，这项研究揭示了疾病控制的潜在途径.特别值得注意的是鉴定了几种富含膜微域的蛋白质，提供对可能参与发病机制的分子机制的见解。了解这些蛋白质的作用为制定有针对性的疾病控制措施提供了基础。总的来说,这项研究为保护水产养殖业免受腐殖质病带来的挑战提供了希望。
