Abstract：
Simultaneous detection of multiple breast cancer-associated miRNAs significantly raises the accuracy and reliability of early diagnosis. In this work, disposable carbon fiber paper serves as the biosensing interface, linking DNA probes via click chemistry to efficiently capture targets and signals efficiently. DNA probes have multiple recognition domains that trigger a cascade reaction through the helper probes and targets, resulting in two signals output. The signals are centrally encapsulated in the pore of the MIL-88(Fe)-NH2. The signal carriers are directed by signal probes to the recognition domains that correspond to the DNA probes. The biosensor is selective and stable, and it can quantify miRNA-21 and miRNA-155 simultaneously with detection limits of 0.64 and 0.54 fmol/L, respectively. Furthermore, it demonstrates satisfactory performance in tests conducted with normal human serum and cell lysate. Overall, this method makes a satisfactory exploration to realize an inexpensive and sensitive biosensor for multiple biomarkers.
摘要：
同时检测多个乳腺癌相关miRNA显著提高了早期诊断的准确性和可靠性。在这项工作中,一次性碳纤维纸作为生物传感接口，通过点击化学连接DNA探针，以有效地捕获靶标和信号。DNA探针具有多个识别结构域，通过辅助探针和靶标触发级联反应，导致两个信号输出。信号集中封装在MIL-88(Fe)-NH2的孔中。信号载体由信号探针引导至对应于DNA探针的识别结构域。该生物传感器具有选择性和稳定性,它可以同时定量miRNA-21和miRNA-155，检出限为0.64和0.54fmol/L，分别。此外，它在用正常人血清和细胞裂解物进行的测试中证明了令人满意的性能。总的来说,该方法为实现一种廉价、灵敏的多生物标志物生物传感器进行了令人满意的探索。
