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Abstract：
Hepatocellular carcinoma (HCC) is the sixth most common malignant tumor and the third leading cause of cancer death worldwide. Most patients are diagnosed at an advanced stage, and systemic chemotherapy is the preferred treatment modality for advanced HCC. Curcumin (CUR) is a polyphenolic antineoplastic drug with low toxicity obtained from plants. However, its low bioavailability and poor solubility limit its functionality. In this study, radiofrequency- (RF) enhanced responsive nanoflowers (NFs), containing superparamagnetic ferric oxide nanoclusters (Fe3O4 NCs), - CUR layer, - and MnO2 (CUR-Fe@MnO2 NFs), were verified to have a thermal therapeutic effect. Transmission electron microscopy was used to characterize the CUR-Fe@MnO2 NFs, which appeared flower-like with a size of 96.27 nm. The in vitro experimental data showed that RF enhanced the degradation of CUR-Fe@MnO2 NFs to release Mn2+ and CUR. The cytotoxicity test results indicated that after RF heating, the CUR-Fe@MnO2 NFs significantly suppressed HCC cell proliferation. Moreover, CUR-Fe@MnO2 NFs were effective T 1/T 2 contrast agents for molecular magnetic resonance imaging due to the release of Mn2+ and Fe3O4 NCs.
摘要：
肝细胞癌（HCC）是全球第六大最常见的恶性肿瘤和第三大癌症死亡原因。大多数病人被诊断为晚期，全身化疗是晚期肝癌的首选治疗方式。姜黄素（CUR）是从植物中获得的具有低毒性的多酚类抗肿瘤药物。然而,其生物利用度低和溶解性差限制了其功能。在这项研究中,射频(RF)增强响应纳米花(NFs)，含超顺磁性氧化铁纳米团簇（Fe3O4NCs），-CUR层，-和MnO2（CUR-Fe@MnO2NFs），被证实具有热治疗效果。透射电子显微镜用于表征CUR-Fe@MnO2NFs，呈花朵状，尺寸为96.27nm。体外实验数据表明，RF增强了CUR-Fe@MnO2NFs的降解以释放Mn2和CUR。细胞毒性试验结果表明,射频加热后,CUR-Fe@MnO2NFs显著抑制HCC细胞增殖。此外，由于释放Mn2和Fe3O4NCs，CUR-Fe@MnO2NFs是分子磁共振成像的有效T1/T2造影剂。
